CD5L ELISA Kit, Mouse
Temporarily not available outside of China.The online Datasheet here is only a general version, for batch specific information please refer to the hard copy included in the product shipping package.
CD5L ELISA Kit, Mouse: 产品信息
产品名称
CD5L ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
6.82 pg/mL
线性范围
15.63-1000 pg/mL
特异性
Recognizes both recombinant and natural Mouse CD5L
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 206.00 | 415.00 | 818.00 |
| SD | 5.54 | 21.34 | 46.07 |
| CV(%) | 2.7% | 5.1% | 5.6% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 208.00 | 405.00 | 794.00 |
| SD | 4.18 | 10.33 | 18.50 |
| CV(%) | 2.0% | 2.6% | 2.3% |
回收率
The recovery of Mouse CD5L spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 103 |
| Range |
| 94 -109% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 91% |
| 1:4 | 100% |
| 1:8 | 113% |
| 1:16 | 86% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This CD5L ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD5L protein in Serum . It contains recombinant Mouse CD5L, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
CD5L ELISA Kit, Mouse: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Mouse CD5L. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CD5L ELISA Kit, Mouse: 别称
42010 ELISA Kit, Mouse; AAC-11 ELISA Kit, Mouse; AI047839 ELISA Kit, Mouse; Api6 ELISA Kit, Mouse; CT2 ELISA Kit, Mouse; Pdp ELISA Kit, Mouse; Sp-alpha ELISA Kit, Mouse
CD5L 背景信息
CD5L, also known as CD5 antigen-like, is a soluble protein belonging to group B of the scavenger receptor cysteine-rich (SRCR) superfamily and contains three SRCR domains. It is a secreted glycoprotein and expressed by macrophages presentin lymphoid tissues (spleen, lymph node, thymus, and bone marrow). It binds to myelomonocytic and lymphoid cells and may play an important role in the regulation of the innate and adaptive immune systems. CD5L functions as a pattern recognition molecule by binding both lipoteichoic acid (LTA) on Gram positive and lipopolysaccharide (LPS) on Gram negative bacteria. and the SRCR domain 1 of CD5L retains both the LPS and LTA binding activities. In addtion, it is revealed that CD5L seems to play a role as an inhibitor of apoptosis.
全称
CD5 molecule-like
参考文献
- Resnick, D. et al., 1994, Trends Biochem. Sci. 19: 5-8.
- Gebe, J. A. et al., 1997, J. Biol. Chem. 272 (10): 6151–6158.
- Sarrias, M.R. et al., 2004, Crit. Rev. Immunol. 24: 1-37.
- Mukhopadhyay, S. and Gordon, S., 2004, Immunobiology 209: 39-49.
- Sarrias, M. R. et al.,2005, J. Biol. Chem. 280 (42): 35391–35398.
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