CD6 ELISA Kit, Mouse

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CD6 ELISA Kit, Mouse: 产品信息

产品名称
CD6 ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Recombinant protein
灵敏度
7.79 pg/mL
线性范围
10.94-700 pg/mL
特异性
Recognizes both recombinant and natural Mouse CD6
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 98.07 208.19 402.57
SD 3.42 6.25 10.22
CV(%) 3.5% 3.0% 2.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 148.20 289.80 564.80
SD 10.47 31.89 38.64
CV(%) 7.1% 11.0% 6.8%
回收率
The recovery of Mouse CD6 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=4)
Average % Recovery
88
Range
87-90%
线性关系
Supernatant
  Recovery of detected
1:2 104%
1:4 103%
1:8 104%
1:16 96%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This CD6 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD6 protein in Recombinant protein . It contains recombinant Mouse CD6, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD6 ELISA Kit, Mouse: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

CD6 背景信息

T-cell differentiation antigen CD6, also known as TP12 and CD6, is a single-pass type I membrane protein which contains threeSRCR domains. CD6 / TP12 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP12 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM ) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP12 binds to activated leukocyte cell adhesion molecule ( CD166 ), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP12 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.
全称
CD6 molecule
参考文献
  • Joo YS. et al., 2000, Arthritis Rheum. 43 (2): 329-35.
  • Singer NG. et al., 2002, Int Immunol. 14 (6): 585-97.
  • Gimferrer I. et al., 2005, J Immunol. 175 (3): 1406-14.
  • Alonso R. et al., 2010, J Autoimmun. 35 (4): 336-41.
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