CD82 ELISA Kit, Human

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CD82 ELISA Kit, Human: 产品信息

产品名称
CD82 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Recombinant protein
灵敏度
97.61 pg/mL
线性范围
109.38-7000 pg/mL
特异性
Recognizes both recombinant and natural Human CD82
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 945.34 1,976.84 4,207.15
SD 44.94 43.49 197.11
CV(%) 4.8% 2.2% 4.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,069.57 2,104.13 4,637.37
SD 126.33 154.53 340.99
CV(%) 11.8% 7.3% 7.4%
回收率
The recovery of Human CD82 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
94
Range
86 -100%
线性关系
Supernatant
  Recovery of detected
1:2 116%
1:4 98%
1:8 97%
1:16 100%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This CD82 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD82 protein in Recombinant protein . It contains recombinant Human CD82, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD82 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes recombinant Human CD82. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CD82 ELISA Kit, Human: 别称

4F9 ELISA Kit, Human; C33 ELISA Kit, Human; GR15 ELISA Kit, Human; IA4 ELISA Kit, Human; KAI-1 ELISA Kit, Human; KAI1 ELISA Kit, Human; R2 ELISA Kit, Human; SAR2 ELISA Kit, Human; ST6 ELISA Kit, Human; TSPAN27 ELISA Kit, Human

CD82 背景信息

CD82, also known as KAI-1, structurally belongs to tetraspanin family while categorised as metastasis suppressor gene on functional grounds. KAI1/CD82 is localized on cell membrane and form interactions with other tetraspanins, integrins and chemokines which are respectively responsible for cell migration, adhesion and signalling. Downregulation of CD82 expression is associated with the advanced stages of many human cancers and correlates with the acquisition of metastatic potential. Recent studies suggest that complex mechanisms underlie CD82 loss of function, including altered transcriptional regulation, splice variant production and post-translational protein modifications, and indicate a central role for CD82 in controlling metastasis as a 'molecular facilitator'. The loss of KAI1/CD82 expression in invasive and metastatic cancers is due to a complex, epigenetic mechanism that probably involves transcription factors such as NFkappaB, p53, and beta-catenin. A loss of KAI1 expression is also associated with the advanced stages of many human malignancies and results in the acquisition of invasive and metastatic capabilities by tumour cells. Thus, KAI1/CD82 is regarded as a wide-spectrum tumor metastasis suppressor.
全称
CD82 molecule
参考文献
  • Malik FA, et al. (2009) KAI-1/CD82, the molecule and clinical implication in cancer and cancer metastasis. Histol Histopathol. 24(4): 519-30.
  • Liu WM, et al. (2006) KAI1/CD82, a tumor metastasis suppressor. Cancer Lett. 240(2): 183-94.
  • Tonoli H, et al. (2006) CD82 metastasis suppressor gene: a potential target for new therapeutics? Trends Mol Med. 11(12): 563-70.
  • Jackson P, et al. (2005) KAI1 tetraspanin and metastasis suppressor. Int J Biochem Cell Biol. 37(3): 530-4.
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