CD86 ELISA Kit, Human

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CD86 ELISA Kit, Human: 产品信息

产品名称
CD86 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
2.68 pg/mL
线性范围
6.25-400 pg/mL
特异性
Recognizes both recombinant and natural Human CD86
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 69.92 138.07 254.63
SD 3.02 6.74 8.58
CV(%) 4.3% 4.9% 3.4%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 76.14 154.20 296.49
SD 6.35 8.65 13.20
CV(%) 8.3% 5.6% 4.5%
回收率
The recovery of Human CD86 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
91
Range
87-95%
线性关系
Serum
  Recovery of detected
1:2 91%
1:4 81%
1:8 79%
1:16 93%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This CD86 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD86 protein in Serum . It contains recombinant Human CD86, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD86 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human B7-2. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CD86 ELISA Kit, Human: 别称

B7-2 ELISA Kit, Human; B7.2 ELISA Kit, Human; B70 ELISA Kit, Human; CD28LG2 ELISA Kit, Human; LAB72 ELISA Kit, Human

CD86 背景信息

CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B7), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.
全称
CD86 antigen
研究领域
参考文献
  • Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26.
  • Rau FC, et al. (2009) B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion. J Immunol. 183(12): 7661-71.
  • Dai ZS, et al. (2009) Defective expression and modulation of B7-2/CD86 on B cells in B cell chronic lymphocytic leukemia. Int J Hematol. 89(5): 656-63.
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