CD93 ELISA Kit, Rat

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CD93 ELISA Kit, Rat: 产品信息

产品名称
CD93 ELISA Kit, Rat
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
9.56 pg/mL
线性范围
18.75-1200 pg/mL
特异性
Recognizes both recombinant and natural Rat CD93
干扰
Preparations of human CD93 at 50 ng/mL in a mid-range recombinant rat CD93 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 132.00 310.00 614.00
SD 5.25 10.63 14.09
CV(%) 4.0% 3.4% 2.3%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 122.00 314.00 614.00
SD 14.85 12.90 20.00
CV(%) 12.2% 4.1% 3.3%
回收率
The recovery of Rat CD93 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
84
Range
81 -87%
Sample
urine (n=3)
Average % Recovery
84
Range
81 -87%
线性关系
Serum
  Recovery of detected
1:2 97%
1:4 103%
1:8 103%
1:16 112%
Supernatant
  Recovery of detected
1:2 93%
1:4 98%
1:8 109%
1:16 95%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This CD93 ELISA Kit, Rat is an enzyme-linked immunosorbent assay for the quantitative measurement of Rat CD93 protein in Serum . It contains recombinant Rat CD93, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD93 ELISA Kit, Rat: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Rat CD93. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CD93 背景信息

CD93 or C1q receptor 1 (C1qR) is an about 12 kDa O-sialoglycoprotein that within the hematopoietic system is selectively expressed on cells of the myeloid lineage. CD93/C1qR is a highly glycosylated transmembrane protein expressed on monocytes, neutrophils, endothelial cells, and stem cells. CD93 was originally identified as a myeloid cell-surface marker and subsequently associated with an ability to modulate phagocytosis of suboptimally opsonized immunoglobulin G and complement particles in vitro. CD93/C1qR, a receptor expressed during early B-cell development, is reinduced during plasma-cell differentiation. High CD93/CD138 expression was restricted to antibody-secreting cells both in T-dependent and T-independent responses as naive, memory, and germinal-center B cells remained CD93-negative. CD93 was expressed on (pre)plasmablasts/plasma cells, including long-lived plasma cells that showed decreased cell cycle activity, high levels of isotype-switched Ig secretion, and modification of the transcriptional network. CD93 is important for the maintenance of plasma cells in bone marrow niches.
全称
CD93 molecule
参考文献
  • Bohlson SS, et al. (2005) CD93 is rapidly shed from the surface of human myeloid cells and the soluble form is detected in human plasma. J Immunol. 175(2): 1239-47.
  • Norsworthy PJ, et al. (2004) Murine CD93 (C1qRp) contributes to the removal of apoptotic cells in vivo but is not required for C1q-mediated enhancement of phagocytosis. J Immunol. 172(6): 3406-14.
  • Chevrier S, et al. (2009) CD93 is required for maintenance of antibody secretion and persistence of plasma cells in the bone marrow niche. Proc Natl Acad Sci U S A. 106(10): 3895-900.
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