COMP ELISA Kit, Human

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COMP ELISA Kit, Human: 产品信息

产品名称
COMP ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
77.8 pg/mL
线性范围
125-8000 pg/mL
特异性
Recognizes both recombinant and natural Human COMP
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,116.00 2,336.00 4,053.00
SD 156.20 230.73 308.79
CV(%) 14.0% 9.9% 7.6%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,017.00 2,157.00 3,995.00
SD 114.61 202.40 92.47
CV(%) 11.3% 9.4% 2.3%
回收率
The recovery of Human COMP spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=2)
Average % Recovery
87
Range
85 -88%
线性关系
Serum
  Recovery of detected
1:2 95%
1:4 105%
1:8 105%
1:16 118%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This COMP ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human COMP protein in Serum . It contains recombinant Human COMP, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

COMP ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human COMP. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

COMP ELISA Kit, Human: 别称

EDM1 ELISA Kit, Human; EPD1 ELISA Kit, Human; MED ELISA Kit, Human; PSACH ELISA Kit, Human; THBS5 ELISA Kit, Human

COMP 背景信息

Cartilage Oligomeric Matrix Protein (COMP), also referred to as Thrombospondin-5, is a non-collagenous extracellular matrix (ECM) protein and belongs to the subgroup B of the thrombospondin protein family. This protein is expressed primarily in cartilage, ligament, and tendon, and binds to other ECM proteins such as collagen I, II and IX with high affinities depending on the divalent cations Zn2+ or Ni2+. COMP is a secreted glycoprotein that is important for growth plate organization and function. It is suggested to play a role in cell growth and development, and recent studies have revealed the possible mechanism that it protects cells against death by elevating members of the IAP (inhibitor of apoptosis protein) family of survival proteins. Mutations in COMP cause two skeletal dysplasias, pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia (EDM1), and up-regulated expression of COMP are observed in rheumatoid arthritis and certain carcinomas.
全称
cartilage oligomeric matrix protein
参考文献
  • Posey KL, et al. (2004) Role of TSP-5/COMP in pseudoachondroplasia. Int J Biochem Cell Biol. 36(6): 1005-12.
  • Chen FH, et al. (2005) Interaction of cartilage oligomeric matrix protein/thrombospondin 5 with aggrecan. J Biol Chem. 282(34): 24591-8.
  • Posey KL, et al. (2008) The role of cartilage oligomeric matrix protein (COMP) in skeletal disease. Curr Drug Targets. 9(10): 869-77.
  • Tan K, et al. (2009) The crystal structure of the signature domain of cartilage oligomeric matrix protein: implications for collagen, glycosaminoglycan and integrin binding. FASEB J. 23(8): 2490-501.
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