SARS-CoV-2 (2019-nCoV) Inhibitor Screening ELISA Kit

COVID-19 Inhibitor Research [ FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES. ]

Intended Use

The kit applies for the screening of SARS-CoV-2 (2019-nCoV) inhibitors.

Principle of the assay

The principle of the kit is competitive ELISA. The SARS-CoV-2 (2019-nCoV) Inhibitor in the samples competes with ACE2-His (Cat: 10108-H08B) to combine with immobilized SARS-CoV-2 S Protein RBD. The signal color becomes lighter as the content of SARS-CoV-2 Inhibitor increases.

Materials Provided

Components 1 Kit (96 Tests)
SARS-CoV-2 Inhibitor 40 μL
Human ACE2 (His Tag) 1 vial
Anti-His Tag Ab (HRP) 20 μL
Microplate: Pre-coated with SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombiant Protein 1 Plate
20 × Dilution Buffer 5 mL
20 × Wash Buffer 25 mL
Color Reagent A 12.5 mL
Color Reagent B 12.5 mL
Stop Solution 8 mL

Storage

Upon receipt, please store each component at the temperature recommended in datasheet.

Typical Data

This inhibition curve is only for demonstration purposes. A curve should be generated for each assay.

SARS-CoV-2 Inhibitor concentration(nM) mean value OD450nm percent activity(%)
0 1.126  
0.004 1.125 99.9
0.012 1.118 99.3
0.037 1.12 99.5
0.11 1.107 98.3
0.329 1.026 91.1
0.988 0.725 64.4
2.963 0.297 26.4
8.889 0.122 10.8
26.667 0.079 7
80 0.074 6.6

The inhibition curve of SARS-CoV-2 Inhibitor to the binding between RBD and ACE2, IC50=1.34 nM

Application Example


Detect three monoclonal antibodies by SARS-CoV-2
Inhibitor Screening ELISA Kit (Cat# KIT001)

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