The kit applies for detecting the level of anti-SARS-CoV-2 (2019-nCoV) Spike RBD antibodies in mouse serum.
The principle of the kit is indirect ELISA. SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombiant Protein has been pre-coated onto well plate strips. The samples or control antibody are added to the well, after incubation the wells are washed and a horseradish peroxidase conjugated Rabbit anti-Mouse IgG is added, producing an complex "antigen-antibody-labeled antibody". Following a wash, the TMB substrate solution is loaded and color develops in proportion to the amount of antibodies. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm.
|Components||1 Kit (96 Tests)|
|Microplate: Pre-coated with SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombiant Protein||1 Plate|
|HRP-Rabbit anti-Mouse IgG||120 μL|
|Anti-SARS-CoV-2 (2019-nCoV) Spike Antibody (Control)||20 μL|
|20 × Dilution Buffer||5 mL|
|20 × Wash Buffer||25 mL|
|Color Reagent A||12.5 mL|
|Color Reagent B||12.5 mL|
|Stop Solution||8 mL|
Unopened Kit: Store at 2 - 8℃ and the kit is stable for 3 months upon receipt.
Opened/Reconstituted Reagents: Please refer to CoA