SARS-CoV-2 (2019-nCoV) Spike RBD Antibody Titer Assay Kit (Mouse)

Temporarily not available outside of China.

Intended Use

The kit applies for detecting the level of anti-SARS-CoV-2 (2019-nCoV) Spike RBD antibodies in mouse serum.

Principle of the assay

The principle of the kit is indirect ELISA. SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombiant Protein has been pre-coated onto well plate strips. The samples or control antibody are added to the well, after incubation the wells are washed and a horseradish peroxidase conjugated Rabbit anti-Mouse IgG is added, producing an complex "antigen-antibody-labeled antibody". Following a wash, the TMB substrate solution is loaded and color develops in proportion to the amount of antibodies. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm.

Materials Provided

Components 1 Kit (96 Tests)
Microplate: Pre-coated with SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombiant Protein 1 Plate
HRP-Rabbit anti-Mouse IgG 120 μL
Anti-SARS-CoV-2 (2019-nCoV) Spike Antibody (Control) 20 μL
20 × Dilution Buffer 5 mL
20 × Wash Buffer 25 mL
Color Reagent A 12.5 mL
Color Reagent B 12.5 mL
Stop Solution 8 mL

Storage

Unopened Kit: Store at 2 - 8℃ and the kit is stable for 3 months upon receipt.
Opened/Reconstituted Reagents: Please refer to CoA

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