Carboxypeptidase B1 ELISA Kit, Human

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Carboxypeptidase B1 ELISA Kit, Human: 产品信息

产品名称
Carboxypeptidase B1 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
5.30 pg/mL
线性范围
6.25-400 pg/mL
特异性
Recognizes both recombinant and natural Human Carboxypeptidase B1
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 57.45 117.80 243.33
SD 5.59 5.15 15.16
CV(%) 9.7% 4.4% 6.2%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 84.52 147.42 288.98
SD 7.96 14.76 23.11
CV(%) 9.4% 10.0% 8.0%
回收率
The recovery of Human CPB1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
87
Range
83 -93%
线性关系
Serum
  Recovery of detected
1:2 103%
1:4 99%
1:8 96%
1:16 109%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Carboxypeptidase B1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Carboxypeptidase B1 protein in Serum . It contains recombinant Human Carboxypeptidase B1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Carboxypeptidase B1 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CPB1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Carboxypeptidase B1 ELISA Kit, Human: 别称

CPB ELISA Kit, Human; PASP ELISA Kit, Human; PCPB ELISA Kit, Human

Carboxypeptidase B1 背景信息

Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
全称
carboxypeptidase B1 (tissue)
参考文献
  • Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581.
  • Pezzilli, R. et al., 1994, Digestion. 55: 73-77.
  • Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.
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