DC-SIGN ELISA Kit, Human
Temporarily not available outside of China.
Cat: KIT10200
DC-SIGN ELISA Kit, Human: 产品信息
产品名称
DC-SIGN ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
11.8 pg/mL
线性范围
31.25-2000 pg/mL
特异性
Recognizes both recombinant and natural Human DC-SIGN
干扰
Preparations of ICAM2、ICAM3、CLU、ICAM5 and LSP1 at 20 ng/mL in a mid-range recombinant human CD209 control were assayed for interference. No significant interference was observed.
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 0.45 | 0.84 | 1.56 |
| SD | 0.02 | 0.04 | 0.06 |
| CV(%) | 3.9% | 4.5% | 3.9% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 0.36 | 0.78 | 1.45 |
| SD | 0.03 | 0.07 | 0.11 |
| CV(%) | 7.5% | 8.8% | 7.8% |
回收率
The recovery of Human CD209 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 110.53802101782999 |
| Range |
| 109%-114% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 101% |
| 1:4 | 99% |
| 1:8 | 73% |
| 1:16 | 84% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This DC-SIGN ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human DC-SIGN protein in Serum . It contains recombinant Human DC-SIGN, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
DC-SIGN ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural human CD209. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.A sample containing 50 ng/mL of recombinant human CLEC4M reads as 0.7ng/mL(1.4% cross-reactivity).
Preparations of ICAM2、ICAM3、CLU、ICAM5 and LSP1 at 20 ng/mL in a mid-range recombinant human CD209 control were assayed for interference. No significant interference was observed.
Preparations of ICAM2、ICAM3、CLU、ICAM5 and LSP1 at 20 ng/mL in a mid-range recombinant human CD209 control were assayed for interference. No significant interference was observed.
DC-SIGN ELISA Kit, Human: 别称
CD209 ELISA Kit, Human; CDSIGN ELISA Kit, Human; CLEC4L ELISA Kit, Human; DC-SIGN ELISA Kit, Human; DC-SIGN1 ELISA Kit, Human; MGC129965 ELISA Kit, Human
DC-SIGN 背景信息
Dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM-3) grabbing nonintegrin (DC-SIGN), also known as CD209, is a type II transmembrane protein on DCs with a C-type lectin extracellular domain, is capable of binding ICAM-3 on resting T cells in the secondary lymphoid organs, providing the initial contact between these cells during the establishment of cell-mediated immunity. It is not only a pattern recognition receptor but implicated in immunoregulation of DCs. It has an important role in mediating DC adhesion, migration, inflammation, activating primary T cell, triggering immune response and participating in immune escape of pathogens and tumors. DC-SIGN also mediates the capture and internalization of viral, bacterial, and fungal pathogens by dendritic cells, such as HIV-1, Ebola virus, cytomegalovirus, Dengue virus, and hepatitis C virus. DC-SIGN is unique in that it regulates adhesion processes, such as DC trafficking and T-cell synapse formation, as well as antigen capture. Moreover, even though several C-type lectins have been shown to bind HIV-1, DC-SIGN does not only capture HIV-1 but also protects it in early endosomes allowing HIV-1 transport by DC to lymphoid tissues, where it enhances trans infection of T cells.
全称
CD209 molecule
参考文献
- Geijtenbeek TB, et al. (2002) DC-SIGN, a C-type lectin on dendritic cells that unveils many aspects of dendritic cell biology. J Leukoc Biol. 71(6): 921-31.
- Masso M. (2003) DC-SIGN points the way to a novel mechanism for HIV-1 transmission. MedGenMed. 5(2): 2.
- Zhou T, et al. (2006) DC-SIGN and immunoregulation. Cell Mol Immunol. 3(4): 279-83.
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