DDR1 ELISA Kit, Human
Temporarily not available outside of China.
Cat: KIT10730
DDR1 ELISA Kit, Human: 产品信息
产品名称
DDR1 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
23.09 pg/mL
线性范围
46.88-3000 pg/mL
特异性
Recognizes both recombinant and natural Human DDR1
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 287.00 | 764.00 | 2,127.00 |
| SD | 21.96 | 36.42 | 84.74 |
| CV(%) | 7.6% | 4.8% | 4.0% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 331.00 | 819.00 | 1,939.00 |
| SD | 41.86 | 60.80 | 176.90 |
| CV(%) | 12.6% | 7.4% | 9.1% |
回收率
The recovery of Human DDR1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 84 |
| Range |
| 81 -87% |
| Sample |
| urine (n=3) |
| Average % Recovery |
| 84 |
| Range |
| 81 -87% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 106% |
| 1:4 | 97% |
| 1:8 | 97% |
| 1:16 | 102% |
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 93% |
| 1:4 | 98% |
| 1:8 | 109% |
| 1:16 | 95% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This DDR1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human DDR1 protein in Serum . It contains recombinant Human DDR1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
DDR1 ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human DDR1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. Cross-reactivity were observed to be 110% and 24% with 500 pg/mL of recombinant mouse DDR1 and rat DDR1.
DDR1 ELISA Kit, Human: 别称
CAK ELISA Kit, Human; CD167 ELISA Kit, Human; DDR ELISA Kit, Human; EDDR1 ELISA Kit, Human; HGK2 ELISA Kit, Human; MCK10 ELISA Kit, Human; NEP ELISA Kit, Human; NTRK4 ELISA Kit, Human; PTK3 ELISA Kit, Human; PTK3A ELISA Kit, Human; RTK6 ELISA Kit, Human; TRKE ELISA Kit, Human
DDR1 背景信息
Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 1 (MCK1), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK1/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK1/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.
全称
discoidin domain receptor tyrosine kinase 1
Related Pathways
-
p53 Pathway
参考文献
- Hou G, et al. (2001) The discoidin domain receptor tyrosine kinase DDR1 in arterial wound repair. J Clin Invest. 107(6): 727-35.
- Ongusaha PP, et al. (2003) p53 induction and activation of DDR1 kinase counteract p53-mediated apoptosis and influence p53 regulation through a positive feedback loop. EMBO J. 22(6): 1289-301.
- Jönsson M, et al. (2001) Repression of Wnt-5a impairs DDR1 phosphorylation and modifies adhesion and migration of mammary cells. J Cell Sci. 114(11): 2043-53.
