DLL1 ELISA Kit, Human

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DLL1 ELISA Kit, Human: 产品信息

产品名称
DLL1 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Cell supernatant
灵敏度
7.6 pg/mL
线性范围
15.63-1000 pg/mL
特异性
Recognizes both recombinant and natural Human DLL1
干扰
Preparations of the following factors at 100 ng/mL in a mid-range recombinant human DLL1 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 208.00 381.00 790.00
SD 6.46 9.44 25.82
CV(%) 3.1% 2.5% 3.3%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 193.00 375.00 791.00
SD 16.41 13.90 17.15
CV(%) 8.5% 3.7% 2.2%
回收率
The recovery of Human DLL1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
82
Range
80 -85%
Sample
supernatant (n=3)
Average % Recovery
98
Range
87 -104%
线性关系
Serum
  Recovery of detected
1:2 91%
1:4 107%
1:8 93%
1:16 94%
Supernatant
  Recovery of detected
1:2 89%
1:4 94%
1:8 99%
1:16 118%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This DLL1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human DLL1 protein in Serum,Cell supernatant . It contains recombinant Human DLL1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

DLL1 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human DLL1. The factors listed below were prepared at 100 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

DLL1 ELISA Kit, Human: 别称

Delta ELISA Kit, Human; DELTA1 ELISA Kit, Human; DL1 ELISA Kit, Human

DLL1 背景信息

Delta-like protein 1(DLL1), also known as Delta1, a single-pass type I membrane protein which contains one DSL domain and eight EGF-like domains, acts as a ligand for Notch receptors, and positively regulates T-cell development. DLL1 is proteolytically processed in a similar manner to the Notch receptor, and it has been speculated to participate in bidirectional signaling. The proteolytic processing of DLL1 helps achieve an asymmetry in Notch signaling in initially equivalent myogenic cells and helps sustain the balance between differentiation and self-renewal. Interactions between DLL1 and Notch in trans activate the Notch pathway, whereas DLL1 binding to Notch in cis inhibits Notch signaling. DLL1 undergoes proteolytic processing in its extracellular domain by ADAM1. It had been demonstrated that DLL1 represents a substrate for several other members of the ADAM family. In co-transfected cells, DLL1 is constitutively cleaved by ADAM12, and the N-terminal fragment of DLL1 is released to medium. ADAM12-mediated cleavage of DLL1 is cell density-dependent, takes place in cis orientation, and does not require the presence of the cytoplasmic domain of ADAM12. Full-length DLL1, but not its N- or C-terminal proteolytic fragment, co-immunoprecipitates with ADAM12. By using a Notch reporter construct, we show that DLL1 processing by ADAM12 increases Notch signaling in a cell-autonomous manner. Furthermore, ADAM9 and ADAM17 have the ability to process DLL1. In contrast, ADAM15 does not cleave DLL1, although the two proteins still co-immunoprecipitate with each other. During fetal development, DLL1 is an essential Notch ligand in the vascular endothelium of large arteries to activate Notch1 and maintain arterial identity. DLL1-Notch signaling was required for VEGF receptor expression in fetal arteries.
全称
delta-like 1 (Drosophila)
研究领域
参考文献
  • Dyczynska E, et al. (2007) Proteolytic processing of delta-like 1 by ADAM proteases. J Biol Chem. 282(1): 436-44.
  • Sun D, et al. (2008) The role of Delta-like 1 shedding in muscle cell self-renewal and differentiation. J Cell Sci. 121(Pt 22): 3815-23.
  • Srensen I, et al. (2009) DLL1-mediated Notch activation regulates endothelial identity in mouse fetal arteries. Blood. 113(22): 5680-8.
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