E-Cadherin ELISA Kit, Human
Temporarily not available outside of China.
Cat: KIT10204
E-Cadherin ELISA Kit, Human: 产品信息
产品名称
E-Cadherin ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
24 pg/mL
线性范围
46.88-3000 pg/mL
特异性
Recognizes both recombinant and natural Human E-Cadherin
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 0.43 | 0.81 | 1.71 |
| SD | 0.02 | 0.05 | 0.12 |
| CV(%) | 5.7% | 6.7% | 7.0% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 0.53 | 0.93 | 1.89 |
| SD | 0.05 | 0.04 | 0.09 |
| CV(%) | 8.9% | 4.2% | 4.9% |
回收率
The recovery of Human CDH1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 98 |
| Range |
| 94-105% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 94% |
| 1:4 | 93% |
| 1:8 | 99% |
| 1:16 | 114% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This E-Cadherin ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human E-Cadherin protein in Serum . It contains recombinant Human E-Cadherin, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
E-Cadherin ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human E-cad. The factors listed above were prepared at 2000 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
E-Cadherin ELISA Kit, Human: 别称
Arc-1 ELISA Kit, Human; CD324 ELISA Kit, Human; CDH1 ELISA Kit, Human; CDHE ELISA Kit, Human; E-cad ELISA Kit, Human; E-Cadherin ELISA Kit, Human; ECAD ELISA Kit, Human; LCAM ELISA Kit, Human; UVO ELISA Kit, Human
E-Cadherin 背景信息
Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.
全称
cadherin 1, type 1, E-cadherin (epithelial)
Related Pathways
-
Canonical Wnt Pathway -
EGFR Signaling Pathway
参考文献
- Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.
- Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.
- Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.
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