Endoglin/CD105 ELISA Kit, Human
Temporarily not available outside of China.
Cat: KIT10149
Endoglin/CD105 ELISA Kit, Human: 产品信息
产品名称
Endoglin/CD105 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
36.27 pg/mL
线性范围
62.5-4000 pg/mL
特异性
Recognizes both recombinant and natural Human Endoglin/CD105
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 834.00 | 1,647.00 | 3,158.00 |
| SD | 22.58 | 41.64 | 95.14 |
| CV(%) | 2.7% | 2.5% | 3.0% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 827.00 | 1,662.00 | 3,209.00 |
| SD | 42.17 | 92.50 | 184.82 |
| CV(%) | 5.1% | 5.6% | 5.8% |
回收率
The recovery of Human ENG spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 81 |
| Range |
| 79 -83% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 102% |
| 1:4 | 98% |
| 1:8 | 117% |
| 1:16 | 101% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Endoglin/CD105 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Endoglin/CD105 protein in Serum . It contains recombinant Human Endoglin/CD105, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
Endoglin/CD105 ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CD105. The factors listed below were prepared at 50 ng/mL or 100ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Endoglin/CD105 ELISA Kit, Human: 别称
END ELISA Kit, Human; HHT1 ELISA Kit, Human; ORW1 ELISA Kit, Human
Endoglin/CD105 背景信息
Endoglin, also known as CD15, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type II receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.
全称
endoglin
参考文献
- Bellon T., et al.,(1993), Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions. Eur. J. Immunol. 23:2340-2345.
- Humphray S.J., et al., (2004), DNA sequence and analysis of human chromosome 9.Nature 429:369-374.
- Gougos A., et al.,(1990), Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells.J. Biol. Chem. 265:8361-8364.
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