ENTPD5 ELISA Kit, Human

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ENTPD5 ELISA Kit, Human: 产品信息

产品名称
ENTPD5 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
5.02 pg/mL
线性范围
23.44-1500 pg/mL
特异性
Recognizes both recombinant and natural Human ENTPD5
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 260.00 551.00 1,058.00
SD 6.13 7.42 26.65
CV(%) 2.4% 1.3% 2.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 244.00 540.00 1,029.00
SD 25.15 25.61 44.59
CV(%) 10.3% 4.7% 4.3%
回收率
The recovery of Human ENTPD5 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
94
Range
88 -100%
Sample
urine (n=3)
Average % Recovery
84
Range
81 -87%
线性关系
Serum
  Recovery of detected
1:2 98%
1:4 105%
1:8 106%
1:16 97%
Supernatant
  Recovery of detected
1:2 93%
1:4 98%
1:8 109%
1:16 95%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This ENTPD5 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human ENTPD5 protein in Serum . It contains recombinant Human ENTPD5, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

ENTPD5 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human ENTPD5. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. A sample containing 5 ng/mL of recombinant mouse ENTPD5 reads as 0.52 ng/mL(10.4% cross-reactivity).

ENTPD5 ELISA Kit, Human: 别称

CD39L4 ELISA Kit, Human; NTPDase-5 ELISA Kit, Human; PCPH ELISA Kit, Human

ENTPD5 背景信息

Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5), also known as CD39 antigen-like 4, ER-UDPase, Guanosine-diphosphatase ENTPD5, Nucleoside diphosphatase Uridine-diphosphatase ENTPD5. This hydrolase is expressed in response to phosphoinositide 3-kinase (PI3K) signaling. Activation of PI3K results in FOXO phosphorylation by AKT1 and loss of ENTPD5 transcriptional repression. It is Up-regulated in PTEN-deficient cells. Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation.ENTPD5 promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, ENTPD5 constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. ENTPD5 also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. This enzyme Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.
全称
ectonucleoside triphosphate diphosphohydrolase 5
参考文献
  • Villar J, et al. (2009) PCPH/ENTPD5 expression confers to prostate cancer cells resistance against cisplatin-induced apoptosis through protein kinase Calpha-mediated Bcl-2 stabilization. Cancer Res. 69(1): 102-10.
  • Fang M, et al. (2010) The ER UDPase ENTPD5 promotes protein N-glycosylation, the Warburg effect, and proliferation in the PTEN pathway. Cell. 143(5): 711-24.
  • Paez JG, et al. (2001) Identity between the PCPH proto-oncogene and the CD39L4 (ENTPD5) ectonucleoside triphosphate diphosphohydrolase gene. Int J Oncol. 19(6): 1249-54.
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