EPOR ELISA Kit, Human

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EPOR ELISA Kit, Human: 产品信息

产品名称
EPOR ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Lysate
灵敏度
1.51 pg/mL
线性范围
9.38-600 pg/mL
特异性
Recognizes both recombinant and natural Human EPOR
干扰
Preparations of human EPO at 100 ng/mL in a mid-range recombinant human EPOR control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 120.00 231.00 440.00
SD 2.74 6.49 7.55
CV(%) 2.3% 2.8% 1.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 111.00 217.00 421.00
SD 6.46 9.44 14.11
CV(%) 5.8% 4.3% 3.4%
回收率
The recovery of Human EPOR spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
80
Range
75 -82%
Sample
lysate (n=3)
Average % Recovery
82
Range
72 -94%
线性关系
Serum
  Recovery of detected
1:2 100%
1:4 100%
1:8 98%
1:16 76%
Lysate
  Recovery of detected
1:2 9,200%
1:4 9,900%
1:8 10,600%
1:16 10,200%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Sample Lysis Buffer
7. Color Reagent A
8. Color Reagent B
9. Stop Solution
产品概述
This EPOR ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human EPOR protein in Serum,Lysate . It contains recombinant Human EPOR, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

EPOR ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human EPOR. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

EPOR ELISA Kit, Human: 别称

EPO-R ELISA Kit, Human

EPOR 背景信息

Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â… single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.
全称
erythropoietin receptor
参考文献
  • Divoky V, et al. (2002) Mouse surviving solely on human erythropoietin receptor (EpoR): model of human EpoR-linked disease. Blood 99(10): 3873-4.
  • Carruthers SG. (2009) A truncated erythropoietin receptor EPOR-T is associated with hypertension susceptibility. Clin Pharmacol Ther. 86(2): 134-6.
  • Baltaziak M, et al. (2009) Relationships of P53 and Bak with EPO and EPOR in human colorectal cancer. Anticancer Res. 29(10):4151-6.
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