Fas/CD95 ELISA Kit, Human

Temporarily not available outside of China.
1/2
Price:
Size:
Number:

Fas/CD95 ELISA Kit, Human: 产品信息

产品名称
Fas/CD95 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Cell supernatant,Plasma
灵敏度
14.6 pg/mL
线性范围
25-1600 pg/mL
特异性
Recognizes both recombinant and natural Human Fas/CD95
干扰
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human FAS control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 339.45 643.91 1,169.26
SD 9.43 16.72 42.46
CV(%) 2.8% 2.6% 3.6%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 346.00 658.30 1,190.80
SD 4.28 18.45 39.70
CV(%) 1.2% 2.8% 3.3%
回收率
The recovery of Human FAS spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
96
Range
91 -102%
Sample
supernatant (n=3)
Average % Recovery
80
Range
78 -84%
Sample
plasma (n=3)
Average % Recovery
94
Range
85 -100%
线性关系
Serum
  Recovery of detected
1:2 84%
1:4 116%
1:8 114%
1:16 95%
Supernatant
  Recovery of detected
1:2 101%
1:4 103%
1:8 117%
1:16 97%
Plasma
  Recovery of detected
1:2 82%
1:4 118%
1:8 118%
1:16 106%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Fas/CD95 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Fas/CD95 protein in Serum,Cell supernatant,Plasma . It contains recombinant Human Fas/CD95, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Fas/CD95 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human FAS. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Fas/CD95 ELISA Kit, Human: 别称

ALPS1A ELISA Kit, Human; APO-1 ELISA Kit, Human; APT1 ELISA Kit, Human; CD95 ELISA Kit, Human; FAS1 ELISA Kit, Human; FASTM ELISA Kit, Human; TNFRSF6 ELISA Kit, Human

Fas/CD95 背景信息

CD95 (APO-1/Fas) is an important inducer of the extrinsic apoptosis signaling pathway and therapy induced apoptosis of many tumor cells has been linked to the activity of CD95. is a prototype death receptor characterized by the presence of an 8 amino acid death domain in its cytoplasmic tail. This domain is essential for the recruitment of a number of signaling components upon activation by either agonistic anti-CD95 antibodies or cognate CD95 ligand that initiate apoptosis. The complex of proteins that forms upon triggering of CD95 is called the death-inducting signaling complex (DISC). The DISC consists of an adaptor protein and initiator caspases and is essential for induction of apoptosis. CD95 is also crucial for the negative selection of B cells within the germinal center (GC). Impairment of CD95-mediated apoptosis results in defective affinity maturation and the persistence of autoreactive B-cell clones. Changes in the expression of CD95 and/or its ligand CD95L are frequently found in human cancer. The downregulation or mutation of CD95 has been proposed as a mechanism by which cancer cells avoid destruction by the immune system through reduced apoptosis sensitivity. Thus, CD95 has therefore been viewed as a tumor suppressor. CD95 has been reported to be involved in the activation of NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and the alternate pathways for CTL-mediated cytotoxicity. Accordingly, this protein is implicated in the pathogenesis of various malignancies and diseases of the immune system. The CD95/CD95L system was implicated in the etiology of inflammatory bowel disease (IBD) based, primarily, on the finding that CD95 is highly expressed in the intestinal epithelial cells and that epithelial apoptosis is increased in IBD.
全称
Fas cell surface death receptor
研究领域
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • p53 Pathway
    p53 Pathway
  • AMPK Signaling Pathway
    AMPK Signaling Pathway
参考文献
  • Mschen M, et al. (2002) The origin of CD95-gene mutations in B-cell lymphoma. Trends Immunol. 23(2): 75-80.
  • Peter ME, et al. (2003) The CD95(APO-1/Fas) DISC and beyond. Cell Death Differ. 10(1): 26-35.
  • Peter ME, et al. (2005) Does CD95 have tumor promoting activities Biochim Biophys Acta. 1755(1): 25-36.
  • Chen L, et al. (2010) Cell death in the colonic epithelium during inflammatory bowel diseases: CD95/Fas and beyond. Inflamm Bowel Dis. 16(6): 1071-6.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.