Ferritin light chain ELISA Kit, Human

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Ferritin light chain ELISA Kit, Human: 产品信息

产品名称
Ferritin light chain ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
10.47 pg/mL
线性范围
62.5-4000 pg/mL
特异性
Recognizes both recombinant and natural Human Ferritin light chain
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 539.45 1,060.48 2,070.38
SD 14.30 33.85 78.56
CV(%) 2.7% 3.2% 3.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 479.23 905.60 1,904.62
SD 43.09 91.65 218.18
CV(%) 9.0% 10.1% 11.5%
回收率
The recovery of Human FTL spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
91
Range
81 -96%
线性关系
Serum
  Recovery of detected
1:2 98%
1:4 97%
1:8 99%
1:16 101%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Ferritin light chain ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Ferritin light chain protein in Serum . It contains recombinant Human Ferritin light chain, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Ferritin light chain ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Ferritin light chain ELISA Kit, Human: 别称

LFTD ELISA Kit, Human; MGC71996 ELISA Kit, Human; NBIA3 ELISA Kit, Human

Ferritin light chain 背景信息

Ferritin, light polypeptide (FTL) is the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Storage of iron in the tissues occurs in the form of ferritin and hemosiderin. The latter originates from ferritin that has undergone intracellular digestion of its protein shell, leaving the iron core. Ferritin and hemosiderin are components of a continuum. Ferritin has been identified in all types of living organisms: animals, plants, molds, and bacteria. Whithin the protein shell of ferritin, iron is first oxidized to the ferric state for storage as ferric oxyhdroxide. Thus, ferritin removes excess iron from the cell sap where it could otherwise participate in peroxidation mechanisms.
全称
ferritin, light polypeptide
参考文献
  • Munro HN, et al. (1988) The ferritin genes: structure, expression, and regulation. Ann N Y Acad Sci. 526: 113-23.
  • Zhang Y, et al. (2008) Comparative proteomic analysis of human placenta derived from assisted reproductive technology. Proteomics. 8 (20): 4344-56.
  • Lebo RV, et al. (1986) Human ferritin light chain gene sequences mapped to several sorted chromosomes. Hum Genet. 71 (4): 325-8.
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