FGFR2 ELISA Kit, Human
Temporarily not available outside of China.
Cat: KIT10824
FGFR2 ELISA Kit, Human: 产品信息
产品名称
FGFR2 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Recombinant protein
灵敏度
7.15 pg/mL
线性范围
10.94-700 pg/mL
特异性
Recognizes both recombinant and natural Human FGFR2
干扰
Preparations of FGF2、FGF6、FGF7、FGF9、FGF17、FGF21 and LYN at 12 ng/mL in a mid-range recombinant human CSTB control were assayed for interference. No significant interference was observed.
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 87.34 | 184.35 | 456.33 |
| SD | 6.61 | 11.69 | 20.39 |
| CV(%) | 7.6% | 6.3% | 4.5% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 86.39 | 181.45 | 357.20 |
| SD | 8.54 | 14.44 | 17.48 |
| CV(%) | 9.9% | 8.0% | 4.9% |
回收率
The recovery of Human FGFR2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 84 |
| Range |
| 81 -87% |
| Sample |
| supernatant (n=3) |
| Average % Recovery |
| 84 |
| Range |
| 81 -87% |
| Sample |
| urine (n=3) |
| Average % Recovery |
| 84 |
| Range |
| 81 -87% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 93% |
| 1:4 | 98% |
| 1:8 | 109% |
| 1:16 | 95% |
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 93% |
| 1:4 | 98% |
| 1:8 | 109% |
| 1:16 | 95% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This FGFR2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human FGFR2 protein in Recombinant protein . It contains recombinant Human FGFR2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
FGFR2 ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes recombinant human FGFR2. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Preparations of FGF2、FGF6、FGF7、FGF9、FGF17、FGF21 and LYN at 12 ng/mL in a mid-range recombinant human CSTB control were assayed for interference. No significant interference was observed.
Preparations of FGF2、FGF6、FGF7、FGF9、FGF17、FGF21 and LYN at 12 ng/mL in a mid-range recombinant human CSTB control were assayed for interference. No significant interference was observed.
FGFR2 ELISA Kit, Human: 别称
BBDS ELISA Kit, Human; BEK ELISA Kit, Human; BFR-1 ELISA Kit, Human; CD332 ELISA Kit, Human; CEK3 ELISA Kit, Human; CFD1 ELISA Kit, Human; ECT1 ELISA Kit, Human; JWS ELISA Kit, Human; K-SAM ELISA Kit, Human; KGFR ELISA Kit, Human; TK14 ELISA Kit, Human; TK25 ELISA Kit, Human
FGFR2 背景信息
FGFR2, also known as CD332, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR2 acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. It is required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. FGFR2 plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. It also promotes cell proliferation in keratinocytes and imature osteoblasts, but promotes apoptosis in differentiated osteoblasts. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal CD332 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1. Defects in CD3322 are the cause of Crouzon syndrome, Jackson-Weiss syndrome, Apert syndrome, Pfeiffer syndrome, Beare-Stevenson cutis gyrata syndrome, familial scaphocephaly syndrome, lacrimo-auriculo-dento-digital syndrome and Antley-Bixler syndrome without genital anomalies or disordered steroidogenesis.
全称
fibroblast growth factor receptor 2
参考文献
- Marie PJ, et al. (2003) Regulation of human cranial osteoblast phenotype by FGF-2, FGFR-2 and BMP-2 signaling. Histol. 17(3):877-85.
- Park WJ, et al. (1996) Novel FGFR2 mutations in Crouzon and Jackson-Weiss syndromes show allelic heterogeneity and phenotypic variability. Hum Mol Genet. 4(7):1229-33.
- Orr-Urtreger A, et al. (1993) Developmental localization of the splicing alternatives of fibroblast growth factor receptor-2 (FGFR2). Dev Biol. 158(2):475-86.
