Galectin-1 ELISA Kit, Human

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Galectin-1 ELISA Kit, Human: 产品信息

产品名称
Galectin-1 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Milk
灵敏度
306.42 pg/mL
线性范围
390.62-25000 pg/mL
特异性
Recognizes both recombinant and natural Human Galectin-1
干扰
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human Galectin1 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 3,269.97 6,985.02 13,273.71
SD 288.68 516.67 616.18
CV(%) 8.8% 7.4% 4.6%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 3,450.67 7,258.49 13,983.04
SD 138.57 332.65 683.33
CV(%) 4.0% 4.6% 4.9%
回收率
The recovery of Human LGALS1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
74
Range
69-77%
Sample
milk (n=3)
Average % Recovery
80
Range
75-88%
线性关系
Serum
  Recovery of detected
1:2 107%
1:4 93%
1:8 94%
1:16 66%
Milk
  Recovery of detected
1:2 100%
1:4 76%
1:8 68%
1:16 121%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Galectin-1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Galectin-1 protein in Serum,Milk . It contains recombinant Human Galectin-1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Galectin-1 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human Galectin1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. Cross-reactivity was observed to be 5% with 50 ng/mL of recombinant mouse Galectin1.

Galectin-1 ELISA Kit, Human: 别称

GAL1 ELISA Kit, Human; Galectin-1 ELISA Kit, Human; GBP ELISA Kit, Human

Galectin-1 背景信息

Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state in order to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumour growth, has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumour progression including cell adhesion, migration and tumour-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.
全称
lectin, galactoside-binding, soluble, 1
参考文献
  • Gaudet AD, et al. (2005) Expression and functions of galectin-1 in sensory and motoneurons. Curr Drug Targets. 6(4): 419-25.
  • Kadoya T, et al. (2006) Structural and functional studies of galectin-1: a novel axonal regeneration-promoting activity for oxidized galectin-1. Curr Drug Targets. 6(4): 375-83.
  • Camby I, et al. (2006) Galectin-1: a small protein with major functions. Glycobiology. 16(11): 137R-157R.
  • Salatino M, et al. (2008) Galectin-1 as a potential therapeutic target in autoimmune disorders and cancer. Expert Opin Biol Ther. 8(1): 45-57.
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