GDF15 ELISA Kit, Human
Temporarily not available outside of China.The online Datasheet here is only a general version, for batch specific information please refer to the hard copy included in the product shipping package.
GDF15 ELISA Kit, Human: 产品信息
产品名称
GDF15 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Urine,Plasma
灵敏度
1.34 pg/mL
线性范围
6.25-400 pg/mL
特异性
Recognizes both recombinant and natural Human GDF15
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 79.79 | 158.69 | 294.66 |
| SD | 3.93 | 5.93 | 8.58 |
| CV(%) | 4.9% | 3.7% | 2.9% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 77.40 | 153.27 | 287.10 |
| SD | 4.95 | 4.15 | 6.32 |
| CV(%) | 6.4% | 2.7% | 2.2% |
回收率
The recovery of Human GDF15 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 94 |
| Range |
| 90-98% |
| Sample |
| urine (n=3) |
| Average % Recovery |
| 106 |
| Range |
| 102-112% |
| Sample |
| plasma (n=3) |
| Average % Recovery |
| 98 |
| Range |
| 92-105% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 93% |
| 1:4 | 99% |
| 1:8 | 101% |
| 1:16 | 95% |
Urine
| Recovery of detected | |
|---|---|
| 1:2 | 94% |
| 1:4 | 106% |
| 1:8 | 113% |
| 1:16 | 102% |
Plasma
| Recovery of detected | |
|---|---|
| 1:2 | 109% |
| 1:4 | 99% |
| 1:8 | 92% |
| 1:16 | 82% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This GDF15 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human GDF15 protein in Serum,Urine,Plasma . It contains recombinant Human GDF15, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
GDF15 ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human GDF15. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
GDF15 ELISA Kit, Human: 别称
GDF-15 ELISA Kit, Human; MIC-1 ELISA Kit, Human; MIC1 ELISA Kit, Human; NAG-1 ELISA Kit, Human; PDF ELISA Kit, Human; PLAB ELISA Kit, Human; PTGFB ELISA Kit, Human
GDF15 背景信息
Growth-differentiation factor 15 (GDF15), also known as MIC-1, is a secreted member of the transforming growth factor (TGF)-β superfamily, as a novel antihypertrophic regulatory factor in the heart. GDF-15 / GDF15 is not expressed in the normal adult heart but is induced in response to conditions that promote hypertrophy and dilated cardiomyopathy and it is expressed highly in liver. GDF-15 / GDF15 has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF-15 / GDF15 is synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein. GDF-15 / GDF15 overexpression arising from an expanded erythroid compartment contributes to iron overload in thalassemia syndromes by inhibiting hepcidin expression.
全称
growth differentiation factor 15
参考文献
- Ago T, et al. (2006) GDF15, a cardioprotective TGF-beta superfamily protein. Circ Res. 98 (3): 294-297.
- Hsiao E, et al. (2000) Characterization of growth-differentiation factor 15, a transforming growth factor beta superfamily member induced following liver injury. Mol Cell Biol. 20 (10): 3742-51.
- Zimmers T, et al. (2005) Growth differentiation factor-15/macrophage inhibitory cytokine-1 induction after kidney and lung injury. Shock. 23 (6): 543-8.
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