GDF15 ELISA Kit, Human

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GDF15 ELISA Kit, Human: 产品信息

产品名称
GDF15 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Urine,Plasma
灵敏度
1.34 pg/mL
线性范围
6.25-400 pg/mL
特异性
Recognizes both recombinant and natural Human GDF15
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 79.79 158.69 294.66
SD 3.93 5.93 8.58
CV(%) 4.9% 3.7% 2.9%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 77.40 153.27 287.10
SD 4.95 4.15 6.32
CV(%) 6.4% 2.7% 2.2%
回收率
The recovery of Human GDF15 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
94
Range
90-98%
Sample
urine (n=3)
Average % Recovery
106
Range
102-112%
Sample
plasma (n=3)
Average % Recovery
98
Range
92-105%
线性关系
Serum
  Recovery of detected
1:2 93%
1:4 99%
1:8 101%
1:16 95%
Urine
  Recovery of detected
1:2 94%
1:4 106%
1:8 113%
1:16 102%
Plasma
  Recovery of detected
1:2 109%
1:4 99%
1:8 92%
1:16 82%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This GDF15 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human GDF15 protein in Serum,Urine,Plasma . It contains recombinant Human GDF15, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

GDF15 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human GDF15. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

GDF15 ELISA Kit, Human: 别称

GDF-15 ELISA Kit, Human; MIC-1 ELISA Kit, Human; MIC1 ELISA Kit, Human; NAG-1 ELISA Kit, Human; PDF ELISA Kit, Human; PLAB ELISA Kit, Human; PTGFB ELISA Kit, Human

GDF15 背景信息

Growth-differentiation factor 15 (GDF15), also known as MIC-1, is a secreted member of the transforming growth factor (TGF)-β superfamily, as a novel antihypertrophic regulatory factor in the heart. GDF-15 / GDF15 is not expressed in the normal adult heart but is induced in response to conditions that promote hypertrophy and dilated cardiomyopathy and it is expressed highly in liver. GDF-15 / GDF15 has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF-15 / GDF15 is synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein. GDF-15 / GDF15 overexpression arising from an expanded erythroid compartment contributes to iron overload in thalassemia syndromes by inhibiting hepcidin expression.
全称
growth differentiation factor 15
参考文献
  • Ago T, et al. (2006) GDF15, a cardioprotective TGF-beta superfamily protein. Circ Res. 98 (3): 294-297.
  • Hsiao E, et al. (2000) Characterization of growth-differentiation factor 15, a transforming growth factor beta superfamily member induced following liver injury. Mol Cell Biol. 20 (10): 3742-51.
  • Zimmers T, et al. (2005) Growth differentiation factor-15/macrophage inhibitory cytokine-1 induction after kidney and lung injury. Shock. 23 (6): 543-8.
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