GM-CSF ELISA Kit, Human
Temporarily not available outside of China.
Cat: KIT10015
GM-CSF ELISA Kit, Human: 产品信息
产品名称
GM-CSF ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Cell supernatant
灵敏度
2.04 pg/mL
线性范围
7.81-500 pg/mL
特异性
Recognizes both recombinant and natural Human GM-CSF
干扰
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human GM-CSF control were assayed for interference. No significant interference was observed.
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 74.52 | 152.95 | 304.79 |
| SD | 2.25 | 3.11 | 9.15 |
| CV(%) | 3.0% | 2.0% | 3.0% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 77.80 | 157.37 | 311.78 |
| SD | 3.74 | 8.52 | 12.78 |
| CV(%) | 4.8% | 5.4% | 4.1% |
回收率
The recovery of Human CSF2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| supernatant (n=3) |
| Average % Recovery |
| 101 |
| Range |
| 98-103% |
线性关系
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 103% |
| 1:4 | 97% |
| 1:8 | 74% |
| 1:16 | 72% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This GM-CSF ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human GM-CSF protein in Cell supernatant . It contains recombinant Human GM-CSF, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
GM-CSF ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human GM-CSF. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
GM-CSF ELISA Kit, Human: 别称
CSF2 ELISA Kit, Human; GM-CSF ELISA Kit, Human; GMCSF ELISA Kit, Human
GM-CSF 背景信息
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is one of an array of cytokines with pivotal roles in embryo implantation and subsequent development. Several cell lineages in the reproductive tract and gestational tissues synthesise GM-CSF under direction by ovarian steroid hormones and signalling agents originating in male seminal fluid and the conceptus. The pre-implantation embryo, invading placental trophoblast cells and the abundant populations of leukocytes controlling maternal immune tolerance are all subject to GM-CSF regulation. GM-CSF stimulates the differentiation of hematopoietic progenitors to monocytes and neutrophils, and reduces the risk for febrile neutropenia in cancer patients. GM-CSF also has been shown to induce the differentiation of myeloid dendritic cells (DCs) that promote the development of T-helper type 1 (cellular) immune responses in cognate T cells. The active form of the protein is found extracellularly as a homodimer, and the encoding gene is localized to a related gene cluster at chromosome region 5q31 which is known to be associated with 5q-syndrome and acute myelogenous leukemia. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target. GM-CSF has been utilized in the clinical management of multiple disease processes. Most recently, GM-CSF has been incorporated into the treatment of malignancies as a sole therapy, as well as a vaccine adjuvant. While the benefits of GM-CSF in this arena have been promising, recent reports have suggested the potential for GM-CSF to induce immune suppression and, thus, negatively impact outcomes in the management of cancer patients. GM-CSF deficiency in pregnancy adversely impacts fetal and placental development, as well as progeny viability and growth after birth, highlighting this cytokine as a central maternal determinant of pregnancy outcome with clinical relevance in human fertility.
全称
colony stimulating factor 2 (granulocyte-macrophage)
研究领域
Related Pathways
-
IL17 signaling pathway
参考文献
- Robertson SA. (2007) GM-CSF regulation of embryo development and pregnancy. Cytokine Growth Factor Rev. 18(3-4): 287-98.
- Waller EK. (2007) The role of sargramostim (rhGM-CSF) as immunotherapy. Oncologist. 12 Suppl 2: 22-6.
- Clive KS, et al. (2010) Use of GM-CSF as an adjuvant with cancer vaccines: beneficial or detrimental? Expert Rev Vaccines. 9(5): 519-25.
