gp130/IL6ST ELISA Kit, Mouse

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gp130/IL6ST ELISA Kit, Mouse: 产品信息

产品名称
gp130/IL6ST ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
9.44 pg/mL
线性范围
23.44-1500 pg/mL
特异性
Recognizes both recombinant and natural Mouse gp130/IL6ST
干扰
Preparations of the following factors at 15 ng/mL in a mid-range recombinant Mouse IL6ST control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 250.04 540.16 1,122.34
SD 18.21 27.45 52.24
CV(%) 7.3% 5.1% 4.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 256.29 533.48 976.38
SD 17.52 13.64 44.57
CV(%) 6.8% 2.6% 4.6%
回收率
The recovery of Mouse IL6ST spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
87.8499677315263
Range
87-88%
线性关系
Serum
  Recovery of detected
1:2 108%
1:4 104%
1:8 94%
1:16 93%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This gp130/IL6ST ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse gp130/IL6ST protein in Serum . It contains recombinant Mouse gp130/IL6ST, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

gp130/IL6ST ELISA Kit, Mouse: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Mouse IL6ST. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

gp130/IL6ST ELISA Kit, Mouse: 别称

5133400A03Rik ELISA Kit, Mouse; AA389424 ELISA Kit, Mouse; BB405851 ELISA Kit, Mouse; CD130 ELISA Kit, Mouse; D13Ertd699e ELISA Kit, Mouse; gp130 ELISA Kit, Mouse

gp130/IL6ST 背景信息

Glycoprotein 13 (also known as gp13, IL6ST, IL6-beta or CD13) is a transmembrane protein which is the founding member of the class of all cytokine receptors. CD13/gp13 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD13/gp13 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD13/gp13 plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp13 for initiating signal transmission. CD13/gp13 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD13/gp13 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.
全称
interleukin 6 signal transducer
Related Pathways
  • AKT Signaling Pathway
    AKT Signaling Pathway
  • Jak-Stat Signaling Pathway
    Jak-Stat Signaling Pathway
  • Autophagy Pathway
    Autophagy Pathway
  • IL12 signaling pathway
    IL12 signaling pathway
参考文献
  • Hibi, et al. (1990) Molecular cloning and expression of an IL-6 signal transducer, gp130. Cell. 63 (6): 1149-57.
  • Kim H, et al. (1997) Transmembrane domain of gp130 contributes to intracellular signal transduction in hepatic cells. J Biol Chem. 272 (49): 30741-7.
  • Giordano V, et al. (1997) Shc mediates IL-6 signaling by interacting with gp130 and Jak2 kinase. J Immunol. 158 (9): 4097-103.
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