Granulin ELISA Kit, Human
Temporarily not available outside of China.
Cat: KIT10826
Granulin ELISA Kit, Human: 产品信息
产品名称
Granulin ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
40.62 pg/mL
线性范围
78.13-5000 pg/mL
特异性
Recognizes both recombinant and natural Human Granulin
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 1,086.00 | 2,208.00 | 4,440.00 |
| SD | 45.17 | 98.64 | 176.45 |
| CV(%) | 4.2% | 4.5% | 4.0% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 1,055.50 | 2,051.20 | 4,280.00 |
| SD | 50.68 | 101.69 | 159.11 |
| CV(%) | 4.8% | 5.0% | 3.7% |
回收率
The recovery of Human GRN spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 100 |
| Range |
| 97-104% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 80% |
| 1:4 | 96% |
| 1:8 | 104% |
| 1:16 | 108% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Granulin ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Granulin protein in Serum . It contains recombinant Human Granulin, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
Granulin ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human GRN. Mouse GRN was prepared at 200 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Granulin ELISA Kit, Human: 别称
CLN11 ELISA Kit, Human; GEP ELISA Kit, Human; GP88 ELISA Kit, Human; Granulin ELISA Kit, Human; PCDGF ELISA Kit, Human; PEPI ELISA Kit, Human; PGRN ELISA Kit, Human; Progranulin ELISA Kit, Human
Granulin 背景信息
Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. Granulin expression inhibited Tat transactivation, and tethering experiments showed that this effect was due, at least in part, to a direct action on cyclin T1 in the absence of Tat.
全称
Granulin Precursor
参考文献
- Hoque M, et al. (2003) The growth factor granulin interacts with cyclin T1 and modulates P-TEFb-dependent transcription. Mol Cell Biol. 23(5): 1688-702.
- Bateman A, et al. (1990) Granulins, a novel class of peptide from leukocytes. Biochem Biophys Res Commun. 173(3): 1161-8.
- Trinh DP, et al. (1999) Epithelin/granulin growth factors: extracellular cofactors for HIV-1 and HIV-2 Tat proteins. Biochem Biophys Res Commun. 256(2): 299-306.
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