Granulin ELISA Kit, Mouse

Temporarily not available outside of China.The online Datasheet here is only a general version, for batch specific information please refer to the hard copy included in the product shipping package.

Granulin ELISA Kit, Mouse: 产品信息

产品名称
Granulin ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
10.87 pg/mL
线性范围
78.13-5000 pg/mL
特异性
Recognizes both recombinant and natural Mouse Granulin
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 911.00 1,694.00 3,586.00
SD 21.53 34.58 113.87
CV(%) 2.4% 2.1% 3.2%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 917.00 1,736.00 3,540.00
SD 11.39 86.32 94.66
CV(%) 1.2% 5.0% 2.7%
回收率
The recovery of Mouse GRN spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
102
Range
96 -106%
线性关系
Serum
  Recovery of detected
1:2 102%
1:4 101%
1:8 97%
1:16 81%
Supernatant
  Recovery of detected
1:2 93%
1:4 98%
1:8 109%
1:16 95%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Granulin ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Granulin protein in Serum . It contains recombinant Mouse Granulin, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Granulin ELISA Kit, Mouse: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Mouse GRN. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Granulin ELISA Kit, Mouse: 别称

epithelin ELISA Kit, Mouse; Pgrn ELISA Kit, Mouse

Granulin 背景信息

Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The precursor protein, progranulin, is also called Proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. Granulin expression inhibited Tat transactivation, and tethering experiments showed that this effect was due, at least in part, to a direct action on cyclin T1 in the absence of Tat.
全称
Granulin Precursor
参考文献
  • Hoque M, et al. (2003) The growth factor granulin interacts with cyclin T1 and modulates P-TEFb-dependent transcription. Mol Cell Biol. 23(5): 1688-702.
  • Bateman A, et al. (1990) Granulins, a novel class of peptide from leukocytes. Biochem Biophys Res Commun. 173(3): 1161-8.
  • Trinh DP, et al. (1999) Epithelin/granulin growth factors: extracellular cofactors for HIV-1 and HIV-2 Tat proteins. Biochem Biophys Res Commun. 256(2): 299-306.
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