Influenza H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Kit

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Influenza H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Kit: 产品信息

产品名称
Influenza H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Kit
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Recombinant protein
灵敏度
4.9 pg/mL
线性范围
15.6-1000 pg/mL
特异性
Recognizes both recombinant and natural H7N9 Hemagglutinin/HA
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.21 0.43 0.81
SD 0.00 0.01 0.01
CV(%) 2.4% 1.8% 1.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.21 0.43 0.79
SD 0.01 0.02 0.05
CV(%) 6.0% 4.3% 6.6%
回收率
The recovery of H7N9 HA spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
75
Range
72-76%
线性关系
Supernatant
  Recovery of detected
1:2 102%
1:4 102%
1:8 99%
1:16 84%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Influenza H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of H7N9 Hemagglutinin/HA protein in Recombinant protein . It contains recombinant H7N9 Hemagglutinin/HA, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Influenza H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Kit: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes recombinant influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA. The H7N9 strains listed below were prepared at 5 ng/mL in dilution buffer and assayed for cross-reactivity, the cross reaction in the following table.
The representative strains listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Hemagglutinin/HA 背景信息

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
全称
Harvey rat sarcoma viral oncogene homolog
参考文献
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). ""Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin"". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). ""Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses"". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). ""Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential"". Avian Dis. 40 (2): 425–37
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