Influenza H7N9 Hemagglutinin / HA ELISA Kit

Temporarily not available outside of China.Online Datasheet is a general version, for batch specific information please refer to the hard copy in shipping package.

Influenza H7N9 Hemagglutinin / HA ELISA Kit: 产品信息

Influenza H7N9 Hemagglutinin / HA ELISA Kit
Solid Phase Sandwich ELISA (quantitative)
Recombinant protein
3 pg/mL
15.6-1000 pg/mL
Recognizes both recombinant and natural H7N9 Hemagglutinin/HA
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.22 0.46 0.87
SD 0.00 0.01 0.02
CV(%) 2.2% 1.6% 2.4%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.22 0.46 0.87
SD 0.00 0.01 0.02
CV(%) 2.1% 2.7% 1.8%
The recovery of H7N9 HA spiked to different levels throughout the range of the assay in related matrices was evaluated.
supernatant (n=3)
Average % Recovery
  Recovery of detected
1:2 112%
1:4 110%
1:8 103%
1:16 91%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
This Influenza H7N9 Hemagglutinin / HA ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of H7N9 Hemagglutinin/HA protein in Recombinant protein . It contains recombinant H7N9 Hemagglutinin/HA, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
This ELISA Kit is shipped at ambient temperature.
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Influenza H7N9 Hemagglutinin / HA ELISA Kit: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes most of recombinant H7N9 proteins.Influenza A H7N9 (A/Shanghai/2/2013) Hemagglutinin / HA is used as the standard. Crossreaction with other H7N9 strains are as follows:
The representative strains that belonged to other subtype listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Hemagglutinin/HA 背景信息

The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
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  • White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
  • Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
  • Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018
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