HER3/ERBB3 ELISA Kit, Human

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HER3/ERBB3 ELISA Kit, Human: 产品信息

产品名称
HER3/ERBB3 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
26.79 pg/mL
线性范围
31.25-2000 pg/mL
特异性
Recognizes both recombinant and natural Human HER3/ERBB3
干扰
Preparations of human EGFR, ErbB2 and ErbB4 at 18 ng/mL in a mid-range recombinant human ErbB3 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 463.00 946.00 1,663.00
SD 0.01 0.03 0.04
CV(%) 3.0% 3.6% 2.6%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 428.00 865.00 1,563.00
SD 0.03 0.06 0.11
CV(%) 8.2% 7.3% 7.0%
回收率
The recovery of Human ERBB3 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
121
Range
115-128%
线性关系
Serum
  Recovery of detected
1:2 79%
1:4 101%
1:8 99%
1:16 90%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This HER3/ERBB3 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human HER3/ERBB3 protein in Serum . It contains recombinant Human HER3/ERBB3, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

HER3/ERBB3 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human ErbB3. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

HER3/ERBB3 ELISA Kit, Human: 别称

c-erbB-3 ELISA Kit, Human; c-erbB3 ELISA Kit, Human; EEBB3 ELISA Kit, Human; ErbB-3 ELISA Kit, Human; erbB3-S ELISA Kit, Human; HER3 ELISA Kit, Human; LCCS2 ELISA Kit, Human; MDA-BF-1 ELISA Kit, Human; p180-ErbB3 ELISA Kit, Human; p45-sErbB3 ELISA Kit, Human; p85-sErbB3 ELISA Kit, Human

HER3/ERBB3 背景信息

ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
全称
erb-b2 receptor tyrosine kinase 3
参考文献
  • Kraus M.H., et al.,(1989), Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors. Proc. Natl. Acad. Sci. U.S.A. 86:9193-9197.
  • Plowman G.D., et al., (1990), Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909.
  • Katoh M., et al.,(1993), c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.Biochem. Biophys. Res. Commun. 192:1189-1197.
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