ICAM-1 ELISA Kit, Human
Temporarily not available outside of China.
Cat: KIT10346
ICAM-1 ELISA Kit, Human: 产品信息
产品名称
ICAM-1 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
6.91 pg/mL
线性范围
25-1600 pg/mL
特异性
Recognizes both recombinant and natural Human ICAM-1
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 275.81 | 569.49 | 1,079.28 |
| SD | 9.38 | 18.58 | 46.06 |
| CV(%) | 3.4% | 3.3% | 4.3% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 303.48 | 578.96 | 1,093.58 |
| SD | 20.86 | 20.29 | 125.47 |
| CV(%) | 6.9% | 3.5% | 11.5% |
回收率
The recovery of Human ICAM1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 95 |
| Range |
| 93 -98% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 91% |
| 1:4 | 98% |
| 1:8 | 102% |
| 1:16 | 97% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This ICAM-1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human ICAM-1 protein in Serum . It contains recombinant Human ICAM-1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
ICAM-1 ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human ICAM1. The factors listed above were prepared at 30 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
ICAM-1 ELISA Kit, Human: 别称
BB2 ELISA Kit, Human; CD54 ELISA Kit, Human; ICAM-1 ELISA Kit, Human; P3.58 ELISA Kit, Human
ICAM-1 背景信息
Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.
全称
intercellular adhesion molecule 1
研究领域
参考文献
- Xu H, et al. (2001) The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node. Eur J Immunol. 31(10): 3085-93.
- Terol MJ, et al. (2003) Soluble intercellular adhesion molecule-1 (s-ICAM-1/s-CD54) in diffuse large B-cell lymphoma: association with clinical characteristics and outcome. Ann Oncol. 14(3): 467-74.
- Mendez MP, et al. (2006) Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 290(5): L962-70.
- Lawson C, et al. (2009) ICAM-1 signaling in endothelial cells. Pharmacol Rep. 61(1): 22-32.
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