IGFBP-3 ELISA Kit, Human

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IGFBP-3 ELISA Kit, Human: 产品信息

产品名称
IGFBP-3 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
2 pg/mL
线性范围
12.5-800 pg/mL
特异性
Recognizes both recombinant and natural Human IGFBP-3
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 70.20 122.80 255.60
SD 3.90 2.90 11.80
CV(%) 5.6% 2.4% 4.6%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 179.30 360.40 657.40
SD 6.50 31.90 37.90
CV(%) 3.6% 8.8% 5.8%
回收率
The recovery of Human IGFBP3 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=4)
Average % Recovery
97
Range
80-109%
线性关系
Serum
  Recovery of detected
1:2 99%
1:4 103%
1:8 100%
1:16 98%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This IGFBP-3 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP-3 protein in Serum . It contains recombinant Human IGFBP-3, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IGFBP-3 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IGFBP3. The factors listed above were prepared at 250 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IGFBP-3 ELISA Kit, Human: 别称

BP-53 ELISA Kit, Human; IBP3 ELISA Kit, Human; IGFBP3 ELISA Kit, Human

IGFBP-3 背景信息

The Insulin-like Growth Factor (IGF) signaling system plays a central role in cellular growth, differentiation and proliferation. IGFBP3 is the most abundant IGF binding protein in human serum and has been shown to be a growth inhibitory, apoptosis-inducing molecule, capable of acting via IGF-dependent and IGF-independent mechanisms. It appears to function both by cell cycle blockade and the induction of apoptosis. IGFBP3 can be transported to the nucleus by an importin beta mediated mechanism, where it has been shown to interact with the retinoid X receptor alpha and possibly other nuclear elements. IGFBP3 antiproliferative signalling appears to require an active transforming growth factor beta (TGF-beta) signalling pathway, and IGFBP3 stimulates phosphorylation of the TGF-beta signalling intermediates Smad2 and Smad3. IGFBP3 has IGF-independent roles in inhibiting cell proliferation in cancer cell lines. Nuclear transcription factor, retinoid X receptor (RXR)-alpha, and IGFBP3 functionally interact to reduce prostate tumor growth and prostate-specific antigen in vivo. Several clinical studies have proposed that individuals with IGFBP3 levels in the upper range of normal may have a decreased risk for certain common cancers. This includes evidence of a protective effect against breast cancer, prostate cancer, colorectal cancer, and lung cancer. Moreover, IGFBP3 inhibits insulin-stimulated glucose uptake into adipocytes independent of IGF.
全称
insulin-like growth factor binding protein 3
参考文献
  • Baxter RC. (2001) Signalling pathways involved in antiproliferative effects of IGFBP-3: a review. Mol Pathol. 54(3): 145-8.
  • Butt AJ, et al. (2001) IGFBP-3 and apoptosis--a license to kill? Apoptosis. 6(3): 199-205.
  • Ali O, et al. (2003) Epidemiology and biology of insulin-like growth factor binding protein-3 (IGFBP-3) as an anti-cancer molecule. Horm Metab Res. 35(11-12): 726-33.
  • Yamada PM, et al. (2009) Perspectives in mammalian IGFBP-3 biology: local vs. systemic action. Am J Physiol Cell Physiol. 296(5): C954-76.
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