IGFBP7 ELISA Kit, Human

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IGFBP7 ELISA Kit, Human: 产品信息

产品名称
IGFBP7 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
18.4 pg/mL
线性范围
47-3000 pg/mL
特异性
Recognizes both recombinant and natural Human IGFBP7
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 411.24 853.11 1,878.77
SD 16.58 34.90 109.92
CV(%) 4.0% 4.1% 5.9%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 480.00 1,022.00 2,132.00
SD 60.02 135.85 300.98
CV(%) 12.5% 13.3% 14.1%
回收率
The recovery of Human IGFBP7 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
101
Range
94-107%
线性关系
Serum
  Recovery of detected
1:2 110%
1:4 90%
1:8 81%
1:16 82%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This IGFBP7 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP7 protein in Serum . It contains recombinant Human IGFBP7, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IGFBP7 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IGFBP7. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IGFBP7 ELISA Kit, Human: 别称

AGM ELISA Kit, Human; FSTL2 ELISA Kit, Human; IBP-7 ELISA Kit, Human; IGFBP-7 ELISA Kit, Human; IGFBP-7v ELISA Kit, Human; IGFBPRP1 ELISA Kit, Human; MAC25 ELISA Kit, Human; PSF ELISA Kit, Human; RAMSVPS ELISA Kit, Human; TAF ELISA Kit, Human

IGFBP7 背景信息

Insulin like growth factor binding protein 7 (IGFBP7) is a member of the IGFBP family. It has been identified in colorectal adenocarcinoma (CRC) cell lines. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are clearly distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF, or act in an IGF-independent manner in the prostate. IGFBP7 could inhibit cell growth, decrease soft agar colony formation activity and induce apoptosis in RKO and SW62 cells. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence and IGF affinity.
全称
insulin-like growth factor binding protein 7
参考文献
  • Oh Y, et al. (1996) Synthesis and characterization of insulin-like growth factor-binding protein (IGFBP)-7. Recombinant human mac25 protein specifically binds IGF-I and -II. J Biol Chem. 271(48): 30322-5.
  • Wilson EM, et al. (1997) Generation and characterization of an IGFBP-7 antibody: identification of 31kD IGFBP-7 in human biological fluids and Hs578T human breast cancer conditioned media. J Clin Endocrinol Metab. 82(4): 1301-3.
  • Lin J, et al. (2007) Methylation patterns of IGFBP7 in colon cancer cell lines are associated with levels of gene expression. J Pathol. 212(1): 83-90.
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