IL-16 ELISA Kit, Mouse
Temporarily not available outside of China.
Cat: KIT51303
IL-16 ELISA Kit, Mouse: 产品信息
产品名称
IL-16 ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Cell supernatant
灵敏度
2.97 pg/mL
线性范围
5.17-350 pg/mL
特异性
Recognizes both recombinant and natural Mouse IL-16
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 38.00 | 70.00 | 144.00 |
| SD | 1.13 | 1.69 | 5.13 |
| CV(%) | 3.0% | 2.4% | 3.6% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 36.00 | 75.00 | 148.00 |
| SD | 3.05 | 2.95 | 10.50 |
| CV(%) | 8.4% | 3.9% | 7.1% |
回收率
The recovery of Mouse IL16 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 81 |
| Range |
| 78 -86% |
| Sample |
| supernatant (n=3) |
| Average % Recovery |
| 122 |
| Range |
| 115 -126% |
| Sample |
| urine (n=3) |
| Average % Recovery |
| 84 |
| Range |
| 81 -87% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 71% |
| 1:4 | 85% |
| 1:8 | 115% |
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 128% |
| 1:4 | 106% |
| 1:8 | 95% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This IL-16 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-16 protein in Serum,Cell supernatant . It contains recombinant Mouse IL-16, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
IL-16 ELISA Kit, Mouse: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Mouse IL16. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Cross-reactivity was observed to be 0.46% with 50 ng/mL of recombinant human IL16.
IL-16 ELISA Kit, Mouse: 别称
mKIAA4048 ELISA Kit, Mouse
IL-16 背景信息
全称
interleukin 16
参考文献
- Maciaszek JW, et al. (1997) IL-16 represses HIV-1 promoter activity. J Immunol. 158(1): 5-8.
- Zhou P, et al. (1997) Human CD4+ cells transfected with IL-16 cDNA are resistant to HIV-1 infection: inhibition of mRNA expression. Nat Med. 3(6): 659-64.
- Theodore AC, et al. (1996) CD4 ligand IL-16 inhibits the mixed lymphocyte reaction. J Immunol. 157(5): 1958-64.
