IL-19 ELISA Kit, Human

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IL-19 ELISA Kit, Human: 产品信息

产品名称
IL-19 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Cell supernatant
灵敏度
37.85 pg/mL
线性范围
62.5-4000 pg/mL
特异性
Recognizes both recombinant and natural Human IL-19
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 501.00 963.00 2,094.00
SD 24.72 40.91 144.69
CV(%) 4.9% 4.2% 6.9%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 477.00 962.00 1,975.00
SD 24.01 79.50 200.36
CV(%) 5.0% 8.3% 10.1%
回收率
The recovery of Human IL19 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
77
Range
74-80%
Sample
supernatant (n=3)
Average % Recovery
97
Range
81 -118%
线性关系
Serum
  Recovery of detected
1:2 90%
1:4 98%
1:8 112%
1:16 108%
Supernatant
  Recovery of detected
1:2 112%
1:4 97%
1:8 91%
1:16 93%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This IL-19 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-19 protein in Serum,Cell supernatant . It contains recombinant Human IL-19, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL-19 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL19. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IL-19 ELISA Kit, Human: 别称

IL-10C ELISA Kit, Human; Interleukin-19 ELISA Kit, Human; MDA1 ELISA Kit, Human; NG.1 ELISA Kit, Human; ZMDA1 ELISA Kit, Human

IL-19 背景信息

The molecular features at the IL19 locus may modestly alter the establishment of HIV-1 infection. Interleukin (IL) 19, IL-2 and IL-24 belong to the IL-1 cytokine family and have been identified to play a role in the regulation of epidermal functions and in inflammation. The expression of IL19 in biopsies of patients with active ulcerative colitis was increased compared with patients with quiescent ulcerative colitis and that colitis was attenuated in IL-19-deficient mice. The disruption of the epithelial barrier with dextran sodium sulfate leads to increased IL-19 expression. Attenuated colitis in IL-19-deficient animals was associated with reduced numbers of IL-6-producing macrophages in the inflamed colonic lamina propria. Microbial-driven expression of IL-19 by intestinal macrophages may contribute to the pathogenesis of inflammatory bowel disease.
全称
interleukin 19
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