IL13RA1 ELISA Kit, Human
Temporarily not available outside of China.
Cat: KIT10943
IL13RA1 ELISA Kit, Human: 产品信息
产品名称
IL13RA1 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
22.09 pg/mL
线性范围
46.88-3000 pg/mL
特异性
Recognizes both recombinant and natural Human IL13RA1
干扰
Preparations of IL13 prepared at 30 ng/mL in a mid-range recombinant human IL13RA1 control was assayed for interference. No significant interference was observed.
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 480.00 | 925.00 | 1,710.00 |
| SD | 13.12 | 28.14 | 68.71 |
| CV(%) | 2.7% | 3.0% | 4.0% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 504.00 | 973.00 | 1,955.00 |
| SD | 27.73 | 58.22 | 156.96 |
| CV(%) | 5.5% | 6.0% | 8.0% |
回收率
The recovery of Human IL13Ra1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 85 |
| Range |
| 84 -87% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 91% |
| 1:4 | 109% |
| 1:8 | 118% |
| 1:16 | 117% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This IL13RA1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL13RA1 protein in Serum . It contains recombinant Human IL13RA1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
IL13RA1 ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL13RA1. The factors listed above were prepared at 50 or 100 ng/mL in dilution buffer and assayed for cross-reactivity. No cross -reactivity was observed.
Preparations of IL13 prepared at 30 ng/mL in a mid-range recombinant human IL13RA1 control was assayed for interference. No significant interference was observed.
Preparations of IL13 prepared at 30 ng/mL in a mid-range recombinant human IL13RA1 control was assayed for interference. No significant interference was observed.
IL13RA1 ELISA Kit, Human: 别称
CD213A1 ELISA Kit, Human; IL-13Ra ELISA Kit, Human; NR4 ELISA Kit, Human
IL13RA1 背景信息
Interleukin 13 receptor, alpha 1, also known as IL13RA1/IL-13RA1 and CD213A1 (cluster of differentiation 213A1), is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. IL13RA1/IL-13RA1 serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. IL13RA1/IL-13RA1 binds with low affinity to interleukin-13 (IL13). This subunit together with IL4RA can form a functional receptor for IL13. IL13RA1/IL-13RA1 also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.
全称
interleukin 13 receptor, alpha 1
Related Pathways
-
AKT Signaling Pathway -
Jak-Stat Signaling Pathway -
Autophagy Pathway -
Common G-Chain signaling pathway
参考文献
- Kawakami M, et al. (2002) Mutation and functional analysis of IL-13 receptors in human malignant glioma cells. Oncol Res. 12 (11-12): 459-67.
- Umeshita-Suyama R, et al. (2000) Characterization of IL-4 and IL-13 signals dependent on the human IL-13 receptor alpha chain 1: redundancy of requirement of tyrosine residue for STAT3 activation. Int Immunol. 12 (11): 1499-509.
- He JQ, et al. (2003) Polymorphisms in the IL13, IL13RA1, and IL4RA genes and rate of decline in lung function in smokers. Am J Respir. Cell Mol Biol. 28 (3): 379-85.
