IL17 ELISA Kit, Mouse

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IL17 ELISA Kit, Mouse: 产品信息

产品名称
IL17 ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Cell supernatant
灵敏度
13.78 pg/mL
线性范围
23.44-1500 pg/mL
特异性
Recognizes both recombinant and natural Mouse IL17
干扰
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant mouse IL17A control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 217.42 448.13 926.78
SD 13.15 19.90 44.37
CV(%) 6.1% 4.4% 4.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 186.37 398.84 871.92
SD 24.92 21.72 65.15
CV(%) 13.4% 5.4% 7.5%
回收率
The recovery of Mouse IL17A spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
99
Range
96-103%
线性关系
Supernatant
  Recovery of detected
1:2 105%
1:4 100%
1:8 99%
1:16 104%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This IL17 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL17 protein in Cell supernatant . It contains recombinant Mouse IL17, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL17 ELISA Kit, Mouse: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Mouse IL17A. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IL17 ELISA Kit, Mouse: 别称

Ctla-8 ELISA Kit, Mouse; Ctla8 ELISA Kit, Mouse; IL-17 ELISA Kit, Mouse; IL-17A ELISA Kit, Mouse; Il17 ELISA Kit, Mouse

IL17 背景信息

IL17, also known as IL17a, is a cytokine belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
全称
interleukin 17A
研究领域
Related Pathways
  • IL12 signaling pathway
    IL12 signaling pathway
  • IL17 signaling pathway
    IL17 signaling pathway
参考文献
  • Andoh A, et al. (2002) IL-17 selectively down-regulates TNF-alpha-induced RANTES gene expression in human colonic subepithelial myofibroblasts. J Immunol. 169(4):1683-7.
  • Kotake S, et al. (1999) IL-17 in synovial fluids from patients with rheumatoid arthritis is a potent stimulator of osteoclastogenesis. J Clin Invest. 103(9):1345-52.
  • Laan M, et al. (1999) Neutrophil recruitment by human IL-17 via C-X-C chemokine release in the airways. J Immunol. 162(4):2347-52.
  • Shin HC, et al. (1999) Regulation of IL-17, IFN-gamma and IL-10 in human CD8(+) T cells by cyclic AMP-dependent signal transduction pathway. Cytokine. 10(11):841-50.
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