IL1R1 ELISA Kit, Mouse
Temporarily not available outside of China.The online Datasheet here is only a general version, for batch specific information please refer to the hard copy included in the product shipping package.
IL1R1 ELISA Kit, Mouse: 产品信息
产品名称
IL1R1 ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
17.67 pg/mL
线性范围
62.5-4000 pg/mL
特异性
Recognizes both recombinant and natural Mouse IL1R1
干扰
Preparations of the Human IL1R1, IL1β and Mouse IL1R2 at 50 ng/mL in a mid-range recombinant Mouse IL1R1 control were assayed for interference. No significant interference was observed.
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 786.00 | 1,614.00 | 3,062.00 |
| SD | 24.81 | 33.51 | 72.19 |
| CV(%) | 3.2% | 2.1% | 2.4% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 792.00 | 1,567.00 | 3,021.00 |
| SD | 17.78 | 116.82 | 94.08 |
| CV(%) | 2.2% | 7.5% | 3.1% |
回收率
The recovery of Mouse IL1R1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 84 |
| Range |
| 81 -87% |
| Sample |
| urine (n=3) |
| Average % Recovery |
| 84 |
| Range |
| 81 -87% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 107% |
| 1:4 | 93% |
| 1:8 | 88% |
| 1:16 | 78% |
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 93% |
| 1:4 | 98% |
| 1:8 | 109% |
| 1:16 | 95% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This IL1R1 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL1R1 protein in Serum . It contains recombinant Mouse IL1R1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
IL1R1 ELISA Kit, Mouse: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Mouse IL1R1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. Cross-reactivity was observed to be 21% with 5000 pg/mL of recombinant rat IL1R1.
IL1R1 ELISA Kit, Mouse: 别称
CD121a ELISA Kit, Mouse; CD121b ELISA Kit, Mouse; IL-1R1 ELISA Kit, Mouse; IL-iR ELISA Kit, Mouse; Il1r-1 ELISA Kit, Mouse
IL1R1 背景信息
Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokines induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R ACP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.
全称
interleukin 1 receptor, type I
研究领域
Related Pathways
-
IL1 signaling pathway
参考文献
- Dale M, et al. (1999). "Interleukin-1 receptor cluster: gene organization of IL1R2, IL1R1, IL1RL2 (IL-1Rrp2), IL1RL1 (T1/ST2), and IL18R1 (IL-1Rrp) on human chromosome 2q.". Genomics 57 (1): 177-9.
- Joos L, et al. (2001). "Association of IL-1beta and IL-1 receptor antagonist haplotypes with rate of decline in lung function in smokers.". Thorax 56 (11): 863-6.
- Vigers GP, et al. (1997). "Crystal structure of the type-I interleukin-1 receptor complexed with interleukin-1beta.". Nature 386 (6621): 190-4.
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