IL1R2 ELISA Kit, Human
Temporarily not available outside of China.The online Datasheet here is only a general version, for batch specific information please refer to the hard copy included in the product shipping package.
IL1R2 ELISA Kit, Human: 产品信息
产品名称
IL1R2 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
1.91 pg/mL
线性范围
19.53-1250 pg/mL
特异性
Recognizes both recombinant and natural Human IL1R2
干扰
Preparations of IL1 and IL1RA prepared at 10 ng/mL in a mid-range recombinant human IL1R2 control were assayed for interference. No significant interference was observed.
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 304.00 | 577.00 | 1,093.00 |
| SD | 6.66 | 12.41 | 33.80 |
| CV(%) | 2.2% | 2.2% | 3.1% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 283.00 | 561.00 | 1,049.00 |
| SD | 19.70 | 24.60 | 37.85 |
| CV(%) | 6.7% | 4.4% | 3.6% |
回收率
The recovery of Human IL1R2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=2) |
| Average % Recovery |
| 113 |
| Range |
| 107 -116% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 104% |
| 1:4 | 100% |
| 1:8 | 101% |
| 1:16 | 95% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This IL1R2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL1R2 protein in Serum . It contains recombinant Human IL1R2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
IL1R2 ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL1R2. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross -reactivity was observed.
Preparations of IL1 and IL1RA prepared at 10 ng/mL in a mid-range recombinant human IL1R2 control were assayed for interference. No significant interference was observed.
Preparations of IL1 and IL1RA prepared at 10 ng/mL in a mid-range recombinant human IL1R2 control were assayed for interference. No significant interference was observed.
IL1R2 ELISA Kit, Human: 别称
CD121b ELISA Kit, Human; CDw121b ELISA Kit, Human; IL-1R-2 ELISA Kit, Human; IL-1RT-2 ELISA Kit, Human; IL-1RT2 ELISA Kit, Human; IL1R2c ELISA Kit, Human; IL1RB ELISA Kit, Human
IL1R2 背景信息
Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate the development and maintenance of the inflammatory responses and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors that can bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling, whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand-binding portion comprised of three Ig-like domains, a single transmembrane region, and a short cytoplasmic domain is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.
全称
interleukin 1 receptor, type II
研究领域
参考文献
- Cannon JG, et al. (1997) Interleukin-1 beta, interleukin-1 receptor antagonist, and soluble interleukin-1 receptor type II secretion in chronic fatigue syndrome. J Clin Immunol. 17 (3): 253-61.
- Liu C, et al. (1996) Cloning and characterization of an alternatively processed human type II interleukin-1 receptor mRNA. J Biol Chem. 271 (34): 20965-72.
- Van der Poll T, et al. (1997) Antiinflammatory cytokine responses during clinical sepsis and experimental endotoxemia: sequential measurements of plasma soluble interleukin (IL)-1 receptor type II, IL-10, and IL-13. J Infect Dis. 175 (1): 118-22.
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