IL1R2 ELISA Kit, Rat

Temporarily not available outside of China.

IL1R2 ELISA Kit, Rat: 产品信息

IL1R2 ELISA Kit, Rat
Solid Phase Sandwich ELISA (quantitative)
4.7 pg/mL
15.63-1000 pg/mL
Recognizes both recombinant and natural Rat IL1R2
Preparations of rat IL1B and rat IL1LB at 50 ng/mL in a mid-range recombinant rat IL1R2 control were assayed for interference. No significant interference was observed.
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 174.00 351.00 631.00
SD 5.38 7.91 37.95
CV(%) 3.1% 2.2% 6.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 162.00 336.00 630.00
SD 11.15 10.18 29.05
CV(%) 6.9% 3.0% 4.6%
The recovery of Rat IL1R2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
serum (n=3)
Average % Recovery
92 -110%
  Recovery of detected
1:2 92%
1:4 109%
1:8 99%
1:16 93%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
This IL1R2 ELISA Kit, Rat is an enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL1R2 protein in Serum . It contains recombinant Rat IL1R2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
This ELISA Kit is shipped at ambient temperature.
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL1R2 ELISA Kit, Rat: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Rat IL1R2. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IL1R2 背景信息

Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate development and maintenance of the inflammatory responses, and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling, whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand binding portion comprised of three Ig-like domains, a single transmembrane region, and a short cytoplasmic domain, is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.
interleukin 1 receptor, type II
  • Cannon JG, et al. (1997) Interleukin-1 beta, interleukin-1 receptor antagonist, and soluble interleukin-1 receptor type II secretion in chronic fatigue syndrome. J Clin Immunol. 17 (3): 253-61.
  • Liu C, et al. (1996) Cloning and characterization of an alternatively processed human type II interleukin-1 receptor mRNA. J Biol Chem. 271 (34): 20965-72.
  • Van der Poll T, et al. (1997) Antiinflammatory cytokine responses during clinical sepsis and experimental endotoxemia: sequential measurements of plasma soluble interleukin (IL)-1 receptor type II, IL-10, and IL-13. J Infect Dis. 175 (1): 118-22.
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