IL2 ELISA Kit, Human

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IL2 ELISA Kit, Human: 产品信息

产品名称
IL2 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Cell supernatant,Plasma
灵敏度
8.61 pg/mL
线性范围
18.75-1200 pg/mL
特异性
Recognizes both recombinant and natural Human IL2
干扰
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human IL2 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 169.00 330.00 614.00
SD 5.23 8.29 18.53
CV(%) 3.1% 2.5% 3.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 165.00 337.00 597.00
SD 12.90 29.57 69.64
CV(%) 7.8% 8.8% 11.7%
回收率
The recovery of Human IL2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
76
Range
74 -80%
Sample
supernatant (n=3)
Average % Recovery
99
Range
90 -108%
Sample
plasma (n=3)
Average % Recovery
77
Range
73 -82%
线性关系
Serum
  Recovery of detected
1:2 96%
1:4 100%
1:8 100%
1:16 92%
Supernatant
  Recovery of detected
1:2 103%
1:4 98%
1:8 102%
1:16 109%
Plasma
  Recovery of detected
1:2 95%
1:4 100%
1:8 100%
1:16 89%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This IL2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL2 protein in Serum,Cell supernatant,Plasma . It contains recombinant Human IL2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL2 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL2. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IL2 ELISA Kit, Human: 别称

IL-2 ELISA Kit, Human; Interleukin-2 ELISA Kit, Human; lymphokine ELISA Kit, Human; TCGF ELISA Kit, Human

IL2 背景信息

Interleukin-2, also known as T-cell growth factor, TCGF, Aldesleukin and IL2, is a secreted protein which belongs to theIL-2 family. Interleukin-2 / IL-2 was the first interleukin molecule to be discovered. Interleukin-2 / IL-2 molecule was first purified to homogeneity by immunoaffinity chromatography by Kendall Smith and his team at Dartmouth Medical School. Interleukin-2 / IL-2 was also the first cytokine shown to mediate its effects via a specific IL-2 receptor, and it was also the first interleukin to be cloned and expressed from a complementary DNA (cDNA) library. Interleukin-2 / IL-2 was designated number 2 because Smith's data at the time indicated that IL-1, produced bymacrophages, facilitates IL-2 production by T lymphocytes (T cells).
Interleukin-2 / IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Interleukin-2 / IL-2 is normally produced by the body during an immune response. When environmental substances (molecules or microbes) gain access to the body, these substances (termed antigens) are recognized as foreign by antigen receptors that are expressed on the surface of lymphocytes. Antigen binding to the T cell receptor (TCR) stimulates the secretion of Interleukin-2 / IL-2, and the expression of IL-2 receptors IL-2R. The IL-2 / IL-2R interaction then stimulates the growth, differentiation and survival of antigen-selected cytotoxic T cells via the activation of the expression of specific genes. Interleukin-2 / IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. The World Reference Standard for Interleukin-2 / IL-2 is produced by the National Institute of Biological Standards and Control in the UK. A recombinant form of Interleukin-2 / IL-2 for clinical use is manufactured by Chiron Corporation with the brand name Proleukin. It has been approved by the Food and Drug Administration (FDA) for the treatment of cancers (malignant melanoma, renal cell cancer), and is in clinical trials for the treatment of chronic viral infections, and as a booster (adjuvant) for vaccines. The use of Interleukin-2 / IL-2 in HIV therapy has been found to be ineffective.
全称
interleukin 2
研究领域
Related Pathways
  • Common G-Chain signaling pathway
    Common G-Chain signaling pathway
参考文献
  • Smith KA, et al.,1980,  J. Exp. Med.151 (6): 1551-6. 
  • Smith KA, et al.,1980, Nature. 287 (5785): 853-5.
  • Taniguchi T, et al.,1983, Nature. 302 (5906): 305.
  • Cantrell DA, et al.,1984, Science. 224 (4655): 1312-6. 
  • Smith KA, et al.,1988, Science. 240 (4856): 1169-76.
  • Wang X. et al., 2005, Science 310:1159-63.
  • Stauber D.J. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 2788-93.
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