IL22 ELISA Kit, Human

Temporarily not available outside of China.
1/2
Price:
Size:
Number:

IL22 ELISA Kit, Human: 产品信息

产品名称
IL22 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Urine,Plasma
灵敏度
0.59 pg/mL
线性范围
2.34-150 pg/mL
特异性
Recognizes both recombinant and natural Human IL22
干扰
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human IL22 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 30.14 59.08 111.54
SD 1.12 1.29 4.57
CV(%) 3.7% 2.2% 4.1%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 30.53 62.73 116.77
SD 2.73 1.37 1.90
CV(%) 8.9% 2.2% 1.6%
回收率
The recovery of Human IL22 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
90
Range
88-92%
Sample
urine (n=3)
Average % Recovery
99
Range
97-100%
Sample
plasma (n=3)
Average % Recovery
83
Range
81 -85%
线性关系
Serum
  Recovery of detected
1:2 97%
1:4 103%
1:8 100%
1:16 82%
Urine
  Recovery of detected
1:2 98%
1:4 102%
1:8 102%
1:16 98%
Plasma
  Recovery of detected
1:2 79%
1:4 103%
1:8 118%
1:16 106%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This IL22 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL22 protein in Serum,Urine,Plasma . It contains recombinant Human IL22, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL22 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural human IL22. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IL22 ELISA Kit, Human: 别称

IL-22 ELISA Kit, Human; IL-D110 ELISA Kit, Human; IL-TIF ELISA Kit, Human; ILTIF ELISA Kit, Human; TIFa ELISA Kit, Human; TIFIL-23 ELISA Kit, Human; zcyto18 ELISA Kit, Human

IL22 背景信息

IL22 is a member of a group of cytokines called the IL-1 family or IL-1 superfamily (including IL-19, IL-2, IL-24, and IL-26), a class of potent mediators of cellular inflammatory responses. It shares use of IL-1R2 in cell signaling with other members of this family, IL-1, IL-26, IL-28A/B and IL-29. IL22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL22 along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems. IL22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-1R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). IL22 and IL-1 receptor chains play a role in cellular targeting and signal transduction to selectively initiate and regulate immune responses. IL22 can contribute to immune disease through the stimulation of inflammatory responses, S1s and defensins. IL22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-1. In some contexts, the pro-inflammatory versus tissue-protective functions of IL22 are regulated by the often co-expressed cytokine IL-17A.
全称
interleukin 22
参考文献
  • Pestka S. et al., 2004, Annu Rev Immunol. 22: 929-79.
  • Xie MH. et al., 2000, J Biol Chem. 275 (40): 31335-9.
  • Jones BC. et al., 2008, Structure. 16 (9): 1333-44.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.