IL2RG ELISA Kit, Mouse

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IL2RG ELISA Kit, Mouse: 产品信息

产品名称
IL2RG ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
15.53 pg/mL
线性范围
31.25-2000 pg/mL
特异性
Recognizes both recombinant and natural Mouse IL2RG
干扰
Preparations of the factors listed below at 20 ng/mL in a mid-range recombinant mouse IL2RG control were assayed for interference. No significant interference was observed. Interference was observed with recombinant mouse IL2 ,mouse IL13RA1 and mouse IL13RA2 at concentrations ≥ 2 ng/mL.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.21 0.40 0.75
SD 0.01 0.01 0.02
CV(%) 3.0% 3.6% 2.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.20 0.38 0.75
SD 0.02 0.03 0.06
CV(%) 11.1% 7.9% 8.0%
回收率
The recovery of Mouse IL2RG spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
120.843828715365
Range
115-126%
线性关系
Serum
  Recovery of detected
1:2 82%
1:4 97%
1:8 116%
1:16 105%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This IL2RG ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL2RG protein in Serum . It contains recombinant Mouse IL2RG, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL2RG ELISA Kit, Mouse: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Mouse IL2RG. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IL2RG ELISA Kit, Mouse: 别称

CD132 ELISA Kit, Mouse; gamma(c) ELISA Kit, Mouse; gc ELISA Kit, Mouse; [g]c ELISA Kit, Mouse

IL2RG 背景信息

The common gamma chain (γc) (or CD132), also known as interleukin-2 receptor subunit gamma or IL2RG, is a member of the type I cytokine receptor family expressed on most lymphocyte (white blood cell) populations, and its gene is found on the X-chromosome of mammals. The common gamma chain (γc) (or IL2RG), is a cytokine receptor sub-unit that is common to the receptor complexes for at least six different interleukin receptors: IL-2, IL-4, IL-7, IL-9, IL-15 and interleukin-21 receptor. It is a component of multiple cytokine receptors that are essential for lymphocyte development and function. X-linked severe combined immunodeficiency (XSCID) is a rare and potentially fatal disease caused by mutations of IL2RG, the gene encoding IL2RG. IL2RG was demonstrated to be a component of the IL-4 receptor on the basis of chemical cross-linking data, the ability of IL2RG to augment IL-4 binding affinity. The observation that IL-2R gamma is a functional component of the IL-4 receptor, together with the finding that IL-2R gamma associates with the IL-7 receptor, begins to elucidate why deficiency of this common gamma chain (gamma c) has a profound effect on lymphoid function and development, as seen in X-linked severe combined immunodeficiency.
全称
interleukin 2 receptor, gamma
Related Pathways
  • Common G-Chain signaling pathway
    Common G-Chain signaling pathway
参考文献
  • Russell SM, et al. (1993) Interleukin-2 receptor gamma chain: a functional component of the interleukin-4 receptor. Science. 262 (5141): 1880-3.
  • Miyazaki T, et al. (1994) Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits. Science. 266 (5187): 1045-7.
  • Takeshita T, et al. (1992) Cloning of the gamma chain of the human IL-2 receptor. Science. 257 (5068): 379-82.
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