IL3 ELISA Kit, Human

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IL3 ELISA Kit, Human: 产品信息

产品名称
IL3 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Recombinant protein
灵敏度
2.9 pg/mL
线性范围
6.25-400 pg/mL
特异性
Recognizes both recombinant and natural Human IL3
干扰
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human IL3 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 65.00 132.00 237.00
SD 3.88 6.33 16.77
CV(%) 5.9% 4.8% 7.1%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 72.00 148.00 245.00
SD 5.69 14.53 20.98
CV(%) 7.9% 9.8% 8.6%
回收率
The recovery of Human IL3 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
100
Range
92 -110%
线性关系
Supernatant
  Recovery of detected
1:2 100%
1:4 100%
1:8 108%
1:16 101%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This IL3 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL3 protein in Recombinant protein . It contains recombinant Human IL3, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL3 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL3. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IL3 ELISA Kit, Human: 别称

IL-3 ELISA Kit, Human; Interleukin-3 ELISA Kit, Human; MCGF ELISA Kit, Human; MULTI-CSF ELISA Kit, Human

IL3 背景信息

IL3 (interleukin 3), also known as IL-3, is a potent growth promoting cytokine which belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
全称
interleukin 3
参考文献
  • Meyer CG, et al.. (2011) IL3 variant on chromosomal region 5q31-33 and protection from recurrent malaria attacks. Hum Mol Genet. 20(6):1173-81.
  • Zambrano A, et al. (2010) IL-3 controls tau modifications and protects cortical neurons from neurodegeneration. Curr Alzheimer Res. 7(7):615-24.
  • Dentelli P, et al.. (2011) IL-3 is a novel target to interfere with tumor vasculature. Oncogene. 30(50):4930-40.
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