IL4R ELISA Kit, Human

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IL4R ELISA Kit, Human: 产品信息

产品名称
IL4R ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Cell supernatant
灵敏度
2.6 pg/mL
线性范围
3.125-200 pg/mL
特异性
Recognizes both recombinant and natural Human IL4R
干扰
Preparations of human IL4 at 2 ng/mL in a mid-range recombinant human IL4R control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 42.90 76.50 140.80
SD 1.81 4.88 7.68
CV(%) 4.2% 6.4% 5.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 39.30 75.70 143.50
SD 2.40 3.53 9.12
CV(%) 6.1% 4.7% 6.4%
回收率
The recovery of Human IL4R spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
95
Range
89 -97%
Sample
supernatant (n=3)
Average % Recovery
91
Range
87 -96%
线性关系
Serum
  Recovery of detected
1:2 80%
1:4 102%
1:8 99%
1:16 99%
Supernatant
  Recovery of detected
1:2 100%
1:4 101%
1:8 105%
1:16 98%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This IL4R ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL4R protein in Serum,Cell supernatant . It contains recombinant Human IL4R, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL4R ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL4R. The factors listed below were prepared at 100 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IL4R ELISA Kit, Human: 别称

CD124 ELISA Kit, Human; IL-4RA ELISA Kit, Human; IL4R ELISA Kit, Human; IL4RA ELISA Kit, Human

IL4R 背景信息

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD124, also known as interleukin 4 receptor (IL4R), is a typeⅠ transmembrane protein that can regulate IgE antibody production in B cells through binding to interleukin 4 and interleukin 13 and promote differentiation of Th2 cells through binding to interleukin 4. The membrane-bound form of CD124 can be hydrolyzed to soluble form which can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.
全称
interleukin 4 receptor
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参考文献
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
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