interferon-alpha/IFNA5 ELISA Kit, Human

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interferon-alpha/IFNA5 ELISA Kit, Human: 产品信息

产品名称
interferon-alpha/IFNA5 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
60.7 pg/mL
线性范围
70.31-4500 pg/mL
特异性
Recognizes both recombinant and natural Human interferon-alpha/IFNA5
干扰
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human IFNA5 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 307.00 718.00 1,532.00
SD 23.00 38.90 58.51
CV(%) 7.5% 5.4% 3.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 351.00 762.00 1,595.00
SD 39.53 38.08 54.62
CV(%) 11.3% 5.0% 3.4%
回收率
The recovery of Human IFNA5 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
90
Range
87-95%
线性关系
Serum
  Recovery of detected
1:2 105%
1:4 99%
1:8 96%
1:16 81%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Sample Dilution Buffer Concentrate
7. Color Reagent A
8. Color Reagent B
9. Stop Solution
产品概述
This interferon-alpha/IFNA5 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human interferon-alpha/IFNA5 protein in Serum . It contains recombinant Human interferon-alpha/IFNA5, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

interferon-alpha/IFNA5 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IFNA5. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

interferon-alpha/IFNA5 ELISA Kit, Human: 别称

IFN-alpha ELISA Kit, Human; IFN-alpha-5 ELISA Kit, Human; IFN-alphaG ELISA Kit, Human; INA5 ELISA Kit, Human; INFA5 ELISA Kit, Human; leIF G ELISA Kit, Human; RP11-380P16.5 ELISA Kit, Human

interferon-alpha/IFNA5 背景信息

Interferon, alpha 5 (IFNA5) belongs to the alpha/beta interferon family. IFNA5 is the only IFNA subtype detected in normal liver, while a mixture of subtypes is observed in the liver tissue of patients with chronic hepatitis C. Interferons are produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.
全称
interferon, alpha 5
参考文献
  • Lau JY, et al. (1993) Discrepancy between biochemical and virological responses to interferon-alpha in chronic hepatitis C. Lancet. 342(8881): 1208-9.
  • Kessler DS, et al. (1990) Interferon-alpha regulates nuclear translocation and DNA-binding affinity of ISGF3, a multimeric transcriptional activator. Genes Dev. 4(10): 1753-65.
  • Gutterman JU. Cytokine therapeutics: lessons from interferon alpha. Proc Natl Acad Sci U S A. 91(4): 1198-205.
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