JAM-A/Junctional Adhesion Molecule A ELISA Kit, Human

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JAM-A/Junctional Adhesion Molecule A ELISA Kit, Human: 产品信息

产品名称
JAM-A/Junctional Adhesion Molecule A ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Cell supernatant,Lysate
灵敏度
4.1 pg/mL
线性范围
7.8-500 pg/mL
特异性
Recognizes both recombinant and natural Human JAM-A/Junctional Adhesion Molecule A
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 41.58 90.34 182.15
SD 3.05 3.21 12.01
CV(%) 7.3% 3.6% 6.6%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 44.78 93.30 200.81
SD 4.10 6.90 13.12
CV(%) 9.2% 7.4% 6.5%
回收率
The recovery of Human F11R spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
80
Range
76 -87%
Sample
supernatant (n=3)
Average % Recovery
61
Range
54 -67%
线性关系
Serum
  Recovery of detected
1:2 96%
1:4 105%
1:8 106%
1:16 94%
Supernatant
  Recovery of detected
1:2 94%
1:4 96%
1:8 98%
1:16 102%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This JAM-A/Junctional Adhesion Molecule A ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human JAM-A/Junctional Adhesion Molecule A protein in Serum,Cell supernatant,Lysate . It contains recombinant Human JAM-A/Junctional Adhesion Molecule A, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

JAM-A/Junctional Adhesion Molecule A ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

JAM-A/Junctional Adhesion Molecule A ELISA Kit, Human: 别称

CD321 ELISA Kit, Human; JAM ELISA Kit, Human; JAM1 ELISA Kit, Human; JAMA ELISA Kit, Human; JCAM ELISA Kit, Human; KAT ELISA Kit, Human; PAM-1 ELISA Kit, Human

JAM-A/Junctional Adhesion Molecule A 背景信息

Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.
全称
F11 receptor
参考文献
  • Guglielmi KM, et al. (2007) Reovirus binding determinants in junctional adhesion molecule-A. J Biol Chem. 282(24): 17930-40.
  • Yeung D, et al. (2008) Decreased junctional adhesion molecule-A expression during blood-brain barrier breakdown. Acta Neuropathol. 115(6): 635-42.
  • Ong KL, et al. (2009) Elevated plasma level of soluble F11 receptor/junctional adhesion molecule-A (F11R/JAM-A) in hypertension. Am J Hypertens. 22(5): 500-5.
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