JAML ELISA Kit, Human

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JAML ELISA Kit, Human: 产品信息

产品名称
JAML ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Lysate
灵敏度
172.69 pg/mL
线性范围
312.5-20000 pg/mL
特异性
Recognizes both recombinant and natural Human JAML
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,137.00 2,499.00 7,564.00
SD 34.01 69.13 166.68
CV(%) 3.0% 2.8% 2.2%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,884.00 4,259.00 9,987.00
SD 248.54 442.73 450.54
CV(%) 13.2% 10.4% 4.5%
回收率
The recovery of Human JAML spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
84
Range
81 -87%
Sample
supernatant (n=3)
Average % Recovery
92
Range
80 -105%
Sample
urine (n=3)
Average % Recovery
84
Range
81 -87%
线性关系
Supernatant
  Recovery of detected
1:2 93%
1:4 98%
1:8 109%
1:16 95%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This JAML ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human JAML protein in Lysate . It contains recombinant Human JAML, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

JAML ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human JAML. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

JAML ELISA Kit, Human: 别称

AMICA ELISA Kit, Human; CREA7-1 ELISA Kit, Human; CREA7-4 ELISA Kit, Human; Gm638 ELISA Kit, Human; JAML ELISA Kit, Human

JAML 背景信息

Junctional adhesion molecules (JAMs) are endothelial and epithelial adhesion molecules involved in the recruitment of circulating leukocytes to inflammatory sites. JAML (Junctional adhesion molecule-like), also known as AMICA1 (Adhesion molecule interacting with CXADR antigen 1), a protein related to the JAM family, is restricted to leukocytes and promotes their adhesion to endothelial cells. It contains 2 extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail involved in activation signaling. Monocytic JAML/AMICA1 plays a critical role in regulating monocyte transendothelial migration (TEM) probably via binding to the endothelial coxsackie and adenovirus receptor (CAR) and other tight junction-associated adhesive molecules. The Expression of JAML/AMICA1 is restricted to the hematopoietic tissues with the exception of liver. JAML may function in transmigration of leukocytes through epithelial and endothelial tissues. Expressed at the plasma membrane of polymorphonuclear leukocytes, JAML/AMICA1 also enhances myeloid leukemia cell adhesion to endothelial cells.
全称
adhesion molecule, interacts with CXADR antigen 1
参考文献
  • Moog-Lutz C, et al. (2003) JAML, a novel protein with characteristics of a junctional adhesion molecule, is induced during differentiation of myeloid leukemia cells. Blood. 102(9): 3371-8.
  • Luissint AC, et al. (2008) JAM-L-mediated leukocyte adhesion to endothelial cells is regulated in cis by alpha4beta1 integrin activation. J Cell Biol. 183(6): 1159-73.
  • Guo YL, et al. (2009) Role of junctional adhesion molecule-like protein in mediating monocyte transendothelial migration. Arterioscler Thromb Vasc Biol. 29(1): 75-83.
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