Kallikrein 13 ELISA Kit, Human

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Kallikrein 13 ELISA Kit, Human: 产品信息

产品名称
Kallikrein 13 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Recombinant protein
灵敏度
8.17 pg/mL
线性范围
31.25-2000 pg/mL
特异性
Recognizes both recombinant and natural Human Kallikrein 13
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 303.97 664.43 1,383.32
SD 15.86 28.70 59.77
CV(%) 5.2% 4.3% 4.3%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 342.12 700.38 1,387.70
SD 29.13 37.69 65.17
CV(%) 8.5% 5.4% 4.7%
回收率
The recovery of Human KLK13 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
78.17916438606089
Range
73%-82%
线性关系
Supernatant
  Recovery of detected
1:2 105%
1:4 101%
1:8 99%
1:16 92%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Kallikrein 13 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Kallikrein 13 protein in Recombinant protein . It contains recombinant Human Kallikrein 13, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Kallikrein 13 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes recombinant Human KLK13. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Kallikrein 13 ELISA Kit, Human: 别称

KLK-L4 ELISA Kit, Human; KLKL4 ELISA Kit, Human

Kallikrein 13 背景信息

Tissue kallikrein 13 (hK13), also known as KLK-L4 (kallikrein-like gene 4), is a member of the human tissue kallikrein family of serine proteases having diverse physiological functions in many tissues. The KLK13 gene resides on chromosome 19q13.3-4 along with other 14 members in a gene cluster and shares a high degree of homology. KLK13 is a trypsin-like, secreted serine protease expressed specifically in the testicular tissue including prostate, salivary gland, breast, and testis. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and may play a role in metastasis. KLK13 may be involved in the pathogenesis and/or progression of breast and ovary cancers, and is regarded as a novel cancer biomarker. In addition, KLK13 interacts and forms complexes with several serum protease inhibitors, such as alpha2-macroglobulin, and its expression is regulated by steroid hormones.
全称
kallikrein-related peptidase 13
参考文献
  • Yousef G.M., et al.,(2000), Identification and characterization of KLK-L4, a new kallikrein-like gene that appears to be down-regulated in breast cancer tissues. J. Biol. Chem. 275:11891-11898.
  • Komatsu N., et al., (2003), Expression and localization of tissue kallikrein mRNAs in human epidermis and appendages.J. Invest. Dermatol. 121:542-549.
  • Andres A.M., et al.,(2007), Understanding the accuracy of statistical haplotype inference with sequence data of known phase.Genet. Epidemiol. 31:659-671.
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