Lactoferrin/LTF ELISA Kit, Human

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Lactoferrin/LTF ELISA Kit, Human: 产品信息

产品名称
Lactoferrin/LTF ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
68.09 pg/mL
线性范围
78.13-5000 pg/mL
特异性
Recognizes both recombinant and natural Human Lactoferrin/LTF
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 894.00 1,767.00 3,420.00
SD 0.04 0.07 0.09
CV(%) 4.4% 3.8% 2.6%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 798.00 1,610.00 3,117.00
SD 0.08 0.11 0.18
CV(%) 10.5% 6.9% 5.7%
回收率
The recovery of Human LTF spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
77
Range
71-82%
线性关系
Serum
  Recovery of detected
1:2 105%
1:4 95%
1:8 90%
1:16 107%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Lactoferrin/LTF ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Lactoferrin/LTF protein in Serum . It contains recombinant Human Lactoferrin/LTF, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Lactoferrin/LTF ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human LTF. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Lactoferrin/LTF ELISA Kit, Human: 别称

GIG12 ELISA Kit, Human; HEL110 ELISA Kit, Human; HLF2 ELISA Kit, Human; LF ELISA Kit, Human

Lactoferrin/LTF 背景信息

Lactotransferrin, also known as Lactoferrin, Talalactoferrin and LTF, is a secreted protein which belongs to the transferrin family. Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and lactoferroxin C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. Lactoferrin / LTF is a globular glycoprotein that is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions. Lactoferrin / LTF is also present in secondary granules of PMN and is secreted by some acinar cells. Lactoferrin / LTF can be purified from milk or produced recombinantly. Human colostrum has the highest concentration, followed by human milk, then cow milk. Lactoferrin / LTF is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucoses. In particular, lactoferrin provides antibacterial activity to human infants. Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands.
全称
lactotransferrin
参考文献
  • Sánchez L, et al.,1992, Arch. Dis. Child. 67 (5): 657 - 61.
  • Wakabayashi H, et al., 2000, J. Antimicrob. Chemother. 46 (4): 595-602.
  • Nozaki A, et al., 2003, J. Biol. Chem. 278 (12): 10162-73.
  • Azzam HS, et al., 2007, Liver Int. 27 (1): 17-25.
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