LDLR/LDL Receptor ELISA Kit, Human

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LDLR/LDL Receptor ELISA Kit, Human: 产品信息

产品名称
LDLR/LDL Receptor ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
21.09 pg/mL
线性范围
46.88-3000 pg/mL
特异性
Recognizes both recombinant and natural Human LDLR/LDL Receptor
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 531.00 1,096.00 1,970.00
SD 60.00 66.12 166.17
CV(%) 11.3% 6.0% 8.4%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 493.00 1,070.00 1,952.00
SD 32.67 110.63 102.58
CV(%) 6.6% 10.3% 5.2%
回收率
The recovery of Human LDLR spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=2)
Average % Recovery
88
Range
87 -88%
线性关系
Serum
  Recovery of detected
1:2 99%
1:4 95%
1:8 100%
1:16 105%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This LDLR/LDL Receptor ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human LDLR/LDL Receptor protein in Serum . It contains recombinant Human LDLR/LDL Receptor, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

LDLR/LDL Receptor ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human LDLR. The factors listed above were prepared at 50 or 100 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

LDLR/LDL Receptor ELISA Kit, Human: 别称

FH ELISA Kit, Human; FHC ELISA Kit, Human; LDL R ELISA Kit, Human; LDL Receptor ELISA Kit, Human; LDLCQ2 ELISA Kit, Human

LDLR/LDL Receptor 背景信息

LDL Receptor, also known as LDLR, is a mosaic protein which belongs to the Low density lipoprotein receptor gene family. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDL Receptor consists of 84 amino acids (after removal of signal peptide) and mediates the endocytosis of cholesterol-rich LDL. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. LDL Receptor is a cell-surface receptor that recognizes the apoprotein B1 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants.
全称
low density lipoprotein receptor
参考文献
  • Yamamoto T, et al. (1984) The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell. 39(1): 27-38.
  • Mao B, et al. (2001) LDL-receptor-related protein 6 is a receptor for Dickkopf proteins. Nature. 411(6835): 321-5.
  • Pinson KI, et al. (2000) An LDL-receptor-related protein mediates Wnt signalling in mice. Nature. 407(6803): 535-8.
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