LEC/CCL16 ELISA Kit, Human
Temporarily not available outside of China.
Cat: KIT10491
LEC/CCL16 ELISA Kit, Human: 产品信息
产品名称
LEC/CCL16 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
2.43 pg/mL
线性范围
4.69-300 pg/mL
特异性
Recognizes both recombinant and natural Human LEC/CCL16
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 32.00 | 64.00 | 145.00 |
| SD | 1.17 | 2.51 | 5.53 |
| CV(%) | 4.2% | 4.2% | 3.9% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 43.83 | 69.60 | 156.75 |
| SD | 3.06 | 2.31 | 6.03 |
| CV(%) | 8.8% | 3.7% | 4.3% |
回收率
The recovery of Human CCL16 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 98 |
| Range |
| 80-119% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 93% |
| 1:4 | 102% |
| 1:8 | 105% |
| 1:16 | 113% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This LEC/CCL16 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human LEC/CCL16 protein in Serum . It contains recombinant Human LEC/CCL16, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
LEC/CCL16 ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CCL16. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
LEC/CCL16 ELISA Kit, Human: 别称
CCL16 ELISA Kit, Human; CKb12 ELISA Kit, Human; HCC-4 ELISA Kit, Human; ILINCK ELISA Kit, Human; LCC-1 ELISA Kit, Human; LEC ELISA Kit, Human; LMC ELISA Kit, Human; Mtn-1 ELISA Kit, Human; NCC-4 ELISA Kit, Human; NCC4 ELISA Kit, Human; SCYA16 ELISA Kit, Human; SCYL4 ELISA Kit, Human
LEC/CCL16 背景信息
CCL16, a chemokine poorly characterized at the functional level. Human CCL16 is a member of the CC family, and its gene maps to human chromosome 17q. In the mouse, only a pseudogene has been identified to date. CCL16 is a functional ligand for CCR1, CCR2, CCR5, and CCR8. Recombinant CCL16 demonstrated chemotactic activity on human monocytes and lymphocytes. Based on the ability of human chemokines to exert activity on and bind to murine receptors, the TSA mouse adenocarcinoma cell line was transfected with human CCL16 cDNA and, in comparison with other cytokines, was shown to be the faster inducer of systemic immune response due to massive, prompt infiltration of leukocytes.
全称
chemokine (C-C motif) ligand 16
参考文献
- Guiducci C, Di Carlo E, Parenza M, et al. Intralesional injection of adenovirus encoding CC chemokine ligand 16 inhibits mammary tumor growth and prevents metastatic-induced death after surgical removal of the treated primary tumor[J]. The Journal of Immunology, 2004, 172(7): 4026-4036.
